Disease resistant plant methods and compositions

• US 10,280,433 B1
• Filed: 07/16/2015
• Issued: 05/07/2019
• Est. Priority Date: 07/21/2014
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Baley, George J., Drost, Derek R., Jia, Hongwu, Pan, Yule, Stein, Jeffrey Michael, Xie, Chongqing, Zhou, Hao
• Primary Examiner(s): Stankovic, Bratislav
• Application Number:
  US14/801,618
• Time in Patent Office: 1,391 Days
• Field of Search: None
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/4684 Zea mays [maize]
• CPC Class Codes: C12Q 1/6865 Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention provides methods and compositions for producing elite lines of corn exhibiting anthracnose stalk rot (ASR) resistance. Also provided in the present invention are corn plants exhibiting ASR resistance resulting from such methods, and methods for breeding corn such that the ASR resistance traits may be transferred to a desired genetic background.

Claims

1. A method of obtaining a corn plant with enhanced anthracnose stalk rot resistance, said method comprising:

a) obtaining a DNA sample from at least one corn plant or part thereof from a population of corn plants;

b) detecting in the DNA sample the presence of an anthracnose stalk rot resistance allele, wherein said allele is within 5 cM of a “

C”

corresponding to position 151 of SEQ ID NO;

102, and wherein the “

C”

at said position is associated with enhanced anthracnose stalk rot resistance;

c) selecting at least a first plant comprising said allele and enhanced anthracnose stalk rot resistance compared to a plant lacking said allele;

d) crossing the plant selected in step c) with a second corn plant, wherein said second corn plant lacks or is heterozygous for said anthracnose stalk rot resistance allele;

e) collecting seeds from the cross of step d); and

f) growing at least one progeny corn plant from the seeds of step e);

wherein said progeny corn plant comprises said allele and has enhanced anthracnose stalk rot resistance compared to a corn plant lacking said allele.

2. The method of claim 1, wherein said polymorphism is located in a chromosomal segment flanked by marker loci AY107053 and AY110435.

3. The method of claim 2, wherein said polymorphism is located in a chromosomal segment flanked by marker loci SEQ ID NO:

52 and SEQ ID NO;

55.

4. The method of claim 2, wherein said polymorphism is located in a chromosomal segment flanked by marker loci SEQ ID NO:

4 and SEQ ID NO;

2.

5. The method of claim 4, wherein said chromosomal segment is flanked by marker loci SEQ ID NO:

52 and SEQ ID NO;

8.

6. The method of claim 5, wherein said chromosomal segment is flanked by marker loci SEQ ID NO:

96 and SEQ ID NO;

106.