Electrode array device having an adsorbed porous reaction layer
First Claim
1. A process for forming an electrode array device for synthesis of an oligonucleotide having a plurality of electrodes, where the plurality of electrodes have a top electrode surface, and a porous reaction layer adsorbed to the top electrode surface for improved synthesis quality comprising:
- (a) providing a substrate including a top electrode surface, one or more recesses in the substrate and a plurality of electrodes positioned in the one or more recesses, where each of the plurality of electrodes is electronically connected to a computer control system, where the computer control system is configured to control an addressing system for selecting and controlling one or more individual electrodes within the plurality of electrodes and a source to supply a current, where the source is electronically connected to each of the plurality of electrodes by the addressing system;
(b) contacting a treatment solution to the top electrode surface, where the treatment solution comprises a chemical species that adsorbs onto the top electrode surface and a solvent capable of dissolving the chemical species;
(c) washing off the treatment solution while leaving a layer of the chemical species adsorbed onto the top electrode surface to form a porous reaction layer; and
(d) performing a plurality of electrochemical synthesis reactions to synthesize an oligonucleotide on the porous reaction layer, where the computer control system is configured such that the current generates a reactive species in the one or more individual electrodes within the plurality of electrodes, where the generation of the reactive species in the one or more individual electrodes within the plurality of electrodes forms a virtual flask adjacent the one or more individual electrodes within the plurality of electrodes, where the plurality of electrochemical synthesis reactions occur on the porous reaction layer within the virtual flask.
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Abstract
There is disclosed an electrode array device having an adsorbed porous reaction layer for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose. A method for preparing an electrode array for improved synthesis quality is disclosed. The method comprises a cleaning method and a method of attachment of a reaction layer. The cleaning method comprises a plasma cleaning method and a chemical cleaning method. The reaction layer is attached after cleaning by exposing the microarray to a solution containing the chemical species having at least one hydroxyl group.
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Citations
20 Claims
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1. A process for forming an electrode array device for synthesis of an oligonucleotide having a plurality of electrodes, where the plurality of electrodes have a top electrode surface, and a porous reaction layer adsorbed to the top electrode surface for improved synthesis quality comprising:
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(a) providing a substrate including a top electrode surface, one or more recesses in the substrate and a plurality of electrodes positioned in the one or more recesses, where each of the plurality of electrodes is electronically connected to a computer control system, where the computer control system is configured to control an addressing system for selecting and controlling one or more individual electrodes within the plurality of electrodes and a source to supply a current, where the source is electronically connected to each of the plurality of electrodes by the addressing system; (b) contacting a treatment solution to the top electrode surface, where the treatment solution comprises a chemical species that adsorbs onto the top electrode surface and a solvent capable of dissolving the chemical species; (c) washing off the treatment solution while leaving a layer of the chemical species adsorbed onto the top electrode surface to form a porous reaction layer; and (d) performing a plurality of electrochemical synthesis reactions to synthesize an oligonucleotide on the porous reaction layer, where the computer control system is configured such that the current generates a reactive species in the one or more individual electrodes within the plurality of electrodes, where the generation of the reactive species in the one or more individual electrodes within the plurality of electrodes forms a virtual flask adjacent the one or more individual electrodes within the plurality of electrodes, where the plurality of electrochemical synthesis reactions occur on the porous reaction layer within the virtual flask. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification