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Serum-free chemically defined cell culture medium

  • US 10,287,550 B2
  • Filed: 05/27/2016
  • Issued: 05/14/2019
  • Est. Priority Date: 06/17/2010
  • Status: Active Grant
First Claim
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1. A serum-free chemically defined cell culture medium composition, the composition comprising:

  • a serum free base medium comprising Dulbecco'"'"'s Modified Eagle Medium and MCDB 201 medium;

    a regulated iron source;

    an electron transport activator;

    an antioxidant;

    a steroid;

    5-hydroxytryptamine;

    a human serum albumin;

    a phospholipid growth factor selected from lysophosphatidic acid and sphingosine 1-phosphate;

    a cell growth factor;

    human wingless/integrated 3a; and

    R-spondin-1, said composition adjusted to a pH in a range of about 7.3 to about 7.5.

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