Detection and assay devices and methods of making and using the same

• US 10,288,607 B2
• Filed: 04/01/2014
• Issued: 05/14/2019
• Est. Priority Date: 03/28/2008
• Status: Active Grant

• Current Assignee: Sentilus Holdco, LLC
• Original Assignee: Sentilus Holdco, LLC
• Inventors: Chilkoti, Ashutosh, Hucknall, Angus
• Primary Examiner(s): Bunker, Amy M
• Application Number:
  US14/242,355
• Publication Number:
  US 20140228249A1
• Time in Patent Office: 1,869 Days
• Field of Search: 506 13, 506 9
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.

Claims

1. A method of non-covalently binding a first member of a specific binding pair to a non-fouling polymer layer, comprising:

(a) providing the non-fouling polymer layer, which is at least macroscopically dry and retains water of hydration, wherein said non-fouling polymer layer comprises brush molecules, each brush molecule comprising;

(i) a polymeric stem comprising monomer core groups having at least one protein-resistant head group coupled thereto, wherein said at least one protein-resistant head group comprises oligo(ethylene glycol)methyl methacrylate (OEGMA), and

(ii) a plurality of branches comprising said at least one protein-resistant head group projecting from said stem;

(b) depositing the first member of the specific binding pair on the non-fouling polymer layer without activation or deactivation of the brush molecules, wherein the first member of a specific binding pair is directly, non-covalently bound to the non-fouling polymer layer; and

(c) drying the non-fouling polymer layer containing the non-covalently bound first member of a specific binding pair for a time sufficient for the non-fouling polymer layer to be at least macroscopically dry,

and wherein a second member of the specific binding pair is a constituent of a biological fluid.

2. The method of claim 1, wherein the depositing comprises non-contact printing.

3. The method of claim 1, wherein the depositing comprises direct contact quill printing.

4. The method of claim 1, wherein the depositing comprises dip pen lithography.

5. The method of claim 1, wherein the depositing comprises piezoelectric printing.

6. The method of claim 1, wherein the depositing comprises a technique selected from the group consisting of microprinting, microstamping, and any combination thereof.

7. The method of claim 1, wherein the brush molecules are from 2-200 nanometers (nm) in length.

8. The method of claim 7, wherein the brush molecules are from 2-100 nm in length.

9. The method of claim 1, wherein the first member of the specific binding pair comprises a plurality of different first members of specific binding pairs, the method comprising depositing each different first member of the plurality at a discrete location on the non-fouling polymer layer.

10. The method of claim 7, wherein the brush molecules have a density of up to 500 milligrams per meter².

11. The method of claim 1, wherein the first member of the specific binding pair is selected from the group consisting of a protein, a peptide, an antibody, a receptor, a ligand, an antigen, a nucleotide, a synthetic nucleic acid, a carbohydrate, a lipid, a drug, a natural nucleic acid, a cell, and any combination thereof.

12. The method of claim 1, wherein the first member of the specific binding pair is an antibody.

13. The method of claim 1, wherein the first member of the specific binding pair is an antigen.

14. The method of claim 11, wherein the first member of the specific binding pair is a carbohydrate.

15. The method of claim 12, wherein a cell comprises the antibody.

16. The method of claim 13, wherein a cell comprises the antigen.

17. The method of claim 14, wherein a cell comprises the carbohydrate.

18. The method of claim 1, wherein the biological fluid is selected from the group consisting of blood, blood plasma, serum, saliva, mucus, tear fluid, and urine.

19. The method of claim 18, wherein the biological fluid is blood.

20. The method of claim 18, wherein the biological fluid is blood plasma.

21. The method of claim 18, wherein the biological fluid is saliva.

22. The method of claim 18, wherein the biological fluid is mucus.

23. The method of claim 18, wherein the biological fluid is tear fluid.

24. The method of claim 18, wherein the biological fluid is urine.

25. The method of claim 1, wherein a second member of the specific binding pair is selected from the group consisting of a protein, a peptide, an antibody, a receptor, a ligand, an antigen, a nucleotide, a synthetic nucleic acid, a carbohydrate, a drug, a blood lipoprotein, and a natural nucleic acid.

26. The method of claim 25, wherein the second member of the specific binding pair is an antibody.

27. The method of claim 25, wherein the second member of the specific binding pair is an antigen.

28. The method of claim 25, wherein the second member of the specific binding pair is a blood lipoprotein.

29. The method of claim 25, wherein the second member of the specific binding pair is a carbohydrate.

30. The method of claim 26, wherein a cell comprises the antibody.

31. The method of claim 27, wherein a cell comprises the antigen.

32. The method of claim 28, wherein a cell comprises the blood lipoprotein.

33. The method of claim 29, wherein a cell comprises the carbohydrate.

34. The method of claim 1, wherein a second member of the specific binding pair is selected from the group consisting of a human cytokine, a human angiogenic factor, a cardiac marker, a diabetes marker, an obesity marker, a fungal infection marker, a viral infection marker, and a microbial infection marker.