×

Spectroscopic methods for the detection of glycated hemoglobin

  • US 10,288,628 B2
  • Filed: 04/10/2015
  • Issued: 05/14/2019
  • Est. Priority Date: 04/11/2014
  • Status: Active Grant
First Claim
Patent Images

1. A microfluidics device for determining a ratio of glycated hemoglobin to total hemoglobin, the microfluidics device comprising:

  • a first compartment configured to receive a whole blood sample, the first compartment comprising a blood separation membrane and a waste collection material positioned therebelow and associated therewith, wherein the blood separation membrane traps red blood cells thereon and/or therein, and wherein plasma passes through the blood separation membrane and is wicked away from the blood separation membrane and into the waste collection material;

    a second compartment comprising a negatively charged surface and a waste collection material positioned therebelow and associated therewith, wherein the negatively charged surface traps hemoglobin thereon and/or therein, and wherein other components of lysed red blood cells are not trapped on the negatively charged surface and are wicked away from the negatively charged surface and into the waste collection material, and wherein the negatively charged surface is configured for subjection to spectroscopy to measure an amount of total hemoglobin trapped thereon and/or therein and an amount of glycated hemoglobin or non-glycated hemoglobin trapped thereon and/or therein; and

    an optical read chamber configured for optical interrogation by a spectrometer;

    wherein the blood separation membrane is configured for releasable movement from association with the waste collection material to association with the negatively charged surface in the second compartment;

    wherein the negatively charged surface is configured for releasable movement from association with the blood separation membrane to a position within the optical read chamber, and wherein the negatively charged surface can be subjected to spectroscopy within the optical read chamber for measuring (1) an amount of total hemoglobin present on the negatively charged surface, and (2) an amount of one of glycated hemoglobin or non-glycated hemoglobin present on the negatively charged surface; and

    wherein a labeling agent is associated with or applied to one of the blood separation membrane and the negatively charged surface, wherein the labeling agent is configured to label one of glycated hemoglobin or non-glycated hemoglobin for measurement thereof.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×