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Decellularization method and system and decellularized tissue formed thereby

  • US 10,293,082 B2
  • Filed: 07/23/2015
  • Issued: 05/21/2019
  • Est. Priority Date: 07/23/2014
  • Status: Active Grant
First Claim
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1. A system for decellularizing a tissue segment comprising:

  • a decellularization chamber;

    a first tissue holder that is removably attachable to a first end of a tissue segment, the tissue segment comprising a lumen and a tissue wall surrounding the lumen, the tissue wall having an interior surface facing the lumen and an exterior surface opposite the interior surface;

    a second tissue holder that is removably attachable to a second end of the tissue segment, wherein upon attachment of a tissue segment to the first and second tissue holders and placement of the tissue segment in the decellularization chamber, a flow path is established that passes through the lumen of the tissue segment and contacts the interior surface of the tissue segment;

    a first flow line;

    a second flow line in fluid communication with and downstream of the first flow line, wherein upon assembly of the system with a tissue segment, the flow path sequentially exits the lumen of the tissue segment, passes through the first flow line, passes through the second flow line, enters the decellularization chamber and contacts the exterior surface of the tissue wall;

    a pump configured for pumping a decellularization solution through the flow path; and

    a control system configured to control characteristics of the decellularization solution along the flow path and thereby establish a pressure differential across the tissue wall.

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