Methods of differentiating stem cells by modulating miR-124

• US 10,294,458 B2
• Filed: 08/18/2016
• Issued: 05/21/2019
• Est. Priority Date: 11/30/2012
• Status: Active Grant

• Current Assignee: Accelerated Biosciences Corp.
• Original Assignee: Accelerated Biosciences Corp.
• Inventors: Lee, Jau-Nan, Lee, Tony Tung-Yin, Lee, Yuta
• Primary Examiner(s): Ton, Thaian N
• Assistant Examiner(s): Montanari, David A
• Application Number:
  US15/240,190
• Publication Number:
  US 20170067024A1
• Time in Patent Office: 1,006 Days
• CPC Class Codes: A61K 35/39 Pancreas; Islets of Langerhans Langerhans cells of epidermis A61K35/36
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 11/04 for throat disorders
• CPC Class Codes: A61P 13/00 Drugs for disorders of the urinary system
• CPC Class Codes: A61P 13/02 of urine or of the urinary tract, e.g. urine acidifiers
• CPC Class Codes: A61P 13/10 of the bladder
• CPC Class Codes: A61P 15/02 for disorders of the vagina
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/04 Antipruritics
• CPC Class Codes: A61P 25/02 for peripheral neuropathies
• CPC Class Codes: A61P 27/02 Ophthalmic agents
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/65 MicroRNA
• CPC Class Codes: C12N 2506/025 from extra-embryonic cells, e.g. trophoblast, placenta
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0678 : Stem cells; Progenitor cells; Precursor cells
• CPC Class Codes: C12P 19/34 : Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12P 21/02 : having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: G01N 33/5023 : on expression patterns
• CPC Class Codes: G01N 33/507 : Pancreatic cells
• CPC Class Codes: G01N 33/5073 : Stem cells

Abstract

Provided herein are methods of differentiating stem cells via modulating miR-124, and the differentiated cells thereby. Also provided herein are methods for the treatment of diseases using the differentiated cells.

Claims

1. An isolated pancreatic progenitor cell, wherein the isolated pancreatic progenitor cell expresses insulin and betatrophin.

2. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell further expresses C-peptide.

3. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell further expresses Ngn3.

4. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell is differentiated from a human trophoblast stem cell.

5. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has a normal karyotype compared to the trophoblast stem cell.

6. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has low or absence of CD133 expression compared to the trophoblast stem cell.

7. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has low or absence of CD133 expression compared to the trophoblast stem cell.

8. The isolated pancreatic progenitor cell of claim 1, wherein the isolated pancreatic progenitor cell further expresses C-peptide and Ngn3.