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Glucose sensors and methods of manufacture thereof

  • US 10,294,507 B2
  • Filed: 05/27/2014
  • Issued: 05/21/2019
  • Est. Priority Date: 10/22/2007
  • Status: Active Grant
First Claim
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1. A device comprising:

  • a reference electrode;

    a counter electrode;

    a working electrode;

    the working electrode being disposed in the vicinity of the reference and counter electrode;

    an electrically conducting membrane;

    the electrically conducting membrane being in operative communication with the working electrode;

    an enzyme layer;

    the enzyme layer being in operative communication with the working electrode;

    a semi-permeable membrane;

    the semi-permeable membrane being in operative communication with the working electrode;

    a first layer of hydrogel in operative communication with the working electrode;

    the first layer of hydrogel comprising an amount of stored oxygen;

    wherein the amount of stored oxygen is proportional to a number of freeze-thaw cycles that the hydrogel is subjected to;

    where the number of freeze-thaw cycles is 1 to 7; and

    a second layer of hydrogel in operative communication with the working electrode;

    the second layer of hydrogel comprising tissue response modifying release agents; and

    the second layer of hydrogel further comprising myoglobin.

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