Respiratory infection assay
First Claim
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1. A quantitative uniplex method for detecting the presence of one or more of at least six respiratory infection-causing microorganisms in a sample or detecting the absence of said respiratory infection-causing microorganisms in said sample, said method comprising:
- A) applying a sample to a test card, wherein said test card comprises six discrete wells;
1) a first well that includes a first probe, for detecting the presence or absence of Respiratory syncytial virus (RSV A &
B), wherein the first probe has a nucleic acid sequence that comprises the nucleic acid sequence TAGGCAATGCWGC (SEQ ID NO;
124);
2) a second well that includes a second probe, for detecting the presence or absence of Rhinoviruses, wherein the second probe has a nucleic acid sequence that comprises the nucleic acid sequence TCYGGGAYGGGACCRACTA (SEQ ID NO;
125);
3) a third well that includes a third probe, for detecting the presence or absence of Human Metapneumovirus (hMPV), wherein the third probe has a nucleic acid sequence that comprises the nucleic acid sequence TCAGCACCAGACACACC (SEQ ID NO;
126);
4) a fourth well that includes a fourth probe, for detecting the presence or absence of Influenza B (Flu B Quad), wherein the fourth probe has a nucleic acid sequence that comprises the nucleic acid sequence TCTGGTCATTGGRGCC (SEQ ID NO;
127);
5) a fifth well that includes a fifth probe, for detecting the presence or absence of Influenza A (Flu A CDC DC), wherein the fifth probe has a nucleic acid sequence that comprises the nucleic acid sequence CGCTCACTGGGCACGGT (SEQ ID NO;
128);
6) a sixth well that includes a sixth probe, for detecting the presence or absence of Influenza A subtype H5 (H5 FRET), wherein the sixth probe has a nucleic acid sequence that comprises the nucleic acid sequence AACTGRGTGTTCATTTTGT (SEQ ID NO;
129);
B) allowing nucleic acid present in the sample to contact with the probes within said wells;
C) detecting for the presence of bound nucleic acid complex in which sample nucleic acid has bound to one or more of said probes;
wherein the presence of bound nucleic acid complex confirms that nucleic acid from one or more of said six respiratory infection-causing microorganisms is present within the sample, and wherein the absence of bound nucleic acid complex confirms that nucleic acid from all of said six respiratory infection-causing microorganisms is absent within the sample and wherein the probes comprise a detectable label and/or a detectable tag.
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Abstract
The present invention provides nucleic acid products and corresponding methods for screening a biological sample for the presence of a respiratory infection-causing microorganism.
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Citations
18 Claims
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1. A quantitative uniplex method for detecting the presence of one or more of at least six respiratory infection-causing microorganisms in a sample or detecting the absence of said respiratory infection-causing microorganisms in said sample, said method comprising:
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A) applying a sample to a test card, wherein said test card comprises six discrete wells; 1) a first well that includes a first probe, for detecting the presence or absence of Respiratory syncytial virus (RSV A &
B), wherein the first probe has a nucleic acid sequence that comprises the nucleic acid sequence TAGGCAATGCWGC (SEQ ID NO;
124);2) a second well that includes a second probe, for detecting the presence or absence of Rhinoviruses, wherein the second probe has a nucleic acid sequence that comprises the nucleic acid sequence TCYGGGAYGGGACCRACTA (SEQ ID NO;
125);3) a third well that includes a third probe, for detecting the presence or absence of Human Metapneumovirus (hMPV), wherein the third probe has a nucleic acid sequence that comprises the nucleic acid sequence TCAGCACCAGACACACC (SEQ ID NO;
126);4) a fourth well that includes a fourth probe, for detecting the presence or absence of Influenza B (Flu B Quad), wherein the fourth probe has a nucleic acid sequence that comprises the nucleic acid sequence TCTGGTCATTGGRGCC (SEQ ID NO;
127);5) a fifth well that includes a fifth probe, for detecting the presence or absence of Influenza A (Flu A CDC DC), wherein the fifth probe has a nucleic acid sequence that comprises the nucleic acid sequence CGCTCACTGGGCACGGT (SEQ ID NO;
128);6) a sixth well that includes a sixth probe, for detecting the presence or absence of Influenza A subtype H5 (H5 FRET), wherein the sixth probe has a nucleic acid sequence that comprises the nucleic acid sequence AACTGRGTGTTCATTTTGT (SEQ ID NO;
129);B) allowing nucleic acid present in the sample to contact with the probes within said wells; C) detecting for the presence of bound nucleic acid complex in which sample nucleic acid has bound to one or more of said probes; wherein the presence of bound nucleic acid complex confirms that nucleic acid from one or more of said six respiratory infection-causing microorganisms is present within the sample, and wherein the absence of bound nucleic acid complex confirms that nucleic acid from all of said six respiratory infection-causing microorganisms is absent within the sample and wherein the probes comprise a detectable label and/or a detectable tag. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification