Method for measuring concentration of analyte
First Claim
1. A method for measuring the concentration of an analyte in a liquid sample using a redox enzymes and a potassium ferricyanide as an electron-transfer mediator, which is executed by a biosensor system having a sensor chip comprising a capillary into which a liquid sample is introduced, a plurality of electrodes disposed within the capillary, and a reagent layer that is disposed within the capillary and includes the electron-transfer mediator,the capillary having a height less than the maximum value of the sum of the diffusion distance of the electron-transfer mediator and the diffusion distance of the analyte at an upper temperature limit for which the biosensor system is functional, the plurality of electrodes including a working electrode and a counter electrode and being disposed so as to be opposite each other in a horizontal plane, and the reagent layer being disposed within the capillary and including the electron-transfer mediator,said measurement method comprising:
- a first voltage application step of applying a first voltage to the working electrode and the counter electrode;
a second voltage application step of applying a second voltage of positive polarity to the working electrode and the counter electrode after the first voltage application step,a measurement voltage application step of applying a measurement voltage to the working electrode and the counter electrode after the second voltage application step,a current detection step of detecting the value of current flowing through the liquid sample during the application of the measurement voltage;
a concentration measurement step of measuring the concentration of the analyte on the basis of the current value; and
wherein the second voltage application step of applying the second voltage to the electrodes prior to the detection of the current value, so that the temperature of the liquid sample will have less effect on the measurement results of the concentration measurement step,the second voltage is positive with respect to a ground used when applying the measurement voltage,the second voltage is lower than the measurement voltage,the height of the capillary of the sensor chip is the distance from the working electrode to an opposite face from the working electrode; and
the height of the capillary of the sensor chip is between 49 and 90 μ
m.
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Accused Products
Abstract
A biosensor system can comprise a sensor chip and a measurement device. The sensor chip comprises a capillary and electrodes disposed within the capillary. The height of the capillary is set to be less than the maximum value of the sum of the diffusion distance of an electron-transfer mediator and the diffusion distance of an analyte at the upper limit of the measurement guaranteed temperature of the biosensor system. The measurement device applies an open circuit voltage, a voltage that is lower than during concentration measurement, or the like to the electrodes of the sensor chip.
28 Citations
5 Claims
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1. A method for measuring the concentration of an analyte in a liquid sample using a redox enzymes and a potassium ferricyanide as an electron-transfer mediator, which is executed by a biosensor system having a sensor chip comprising a capillary into which a liquid sample is introduced, a plurality of electrodes disposed within the capillary, and a reagent layer that is disposed within the capillary and includes the electron-transfer mediator,
the capillary having a height less than the maximum value of the sum of the diffusion distance of the electron-transfer mediator and the diffusion distance of the analyte at an upper temperature limit for which the biosensor system is functional, the plurality of electrodes including a working electrode and a counter electrode and being disposed so as to be opposite each other in a horizontal plane, and the reagent layer being disposed within the capillary and including the electron-transfer mediator, said measurement method comprising: -
a first voltage application step of applying a first voltage to the working electrode and the counter electrode; a second voltage application step of applying a second voltage of positive polarity to the working electrode and the counter electrode after the first voltage application step, a measurement voltage application step of applying a measurement voltage to the working electrode and the counter electrode after the second voltage application step, a current detection step of detecting the value of current flowing through the liquid sample during the application of the measurement voltage; a concentration measurement step of measuring the concentration of the analyte on the basis of the current value; and wherein the second voltage application step of applying the second voltage to the electrodes prior to the detection of the current value, so that the temperature of the liquid sample will have less effect on the measurement results of the concentration measurement step, the second voltage is positive with respect to a ground used when applying the measurement voltage, the second voltage is lower than the measurement voltage, the height of the capillary of the sensor chip is the distance from the working electrode to an opposite face from the working electrode; and the height of the capillary of the sensor chip is between 49 and 90 μ
m. - View Dependent Claims (2, 3, 4, 5)
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Specification