Tip overlay for continuous flow spotting apparatus
First Claim
1. A method of depositing at least one substance from a carrier fluid on a deposition surface, the method comprising:
- a) loading a carrier fluid having at least one substance into a first well of a spotter apparatus, wherein the spotter apparatus comprises;
i) a loading surface including the first well and a second well;
ii) an outlet surface, different than the loading surface, including a first opening and a second opening;
iii) a first microconduit fluidly coupling the first well with the first opening;
iv) a second microconduit fluidly coupling the second well with the second opening; and
v) an overlay positioned on the outlet surface, wherein the overlay comprises a deposition channel that fully penetrates through the overlay, wherein the deposition channel is situated to communicate carrier fluid among the first opening, the second opening, and a deposition surface when the overlay is pressed against the deposition surface,b) pressing the overlay against the deposition surface to form a seal between the overlay and deposition surface, andc) flowing the carrier fluid in series from the first well, through the first microconduit to the deposition channel, through the second microconduit and into the second well, wherein the deposition channel directs at least one substance from the carrier fluid onto the deposition surface.
6 Assignments
0 Petitions
Accused Products
Abstract
The present disclosure provides apparatuses, systems, and methods involving a spotter apparatus for depositing a substance from a carrier fluid onto a deposition surface in an ordered array. The spotter apparatus includes a loading surface, including a first well and a second well, and a different outlet surface, including a first opening and a second opening, where a first microconduit fluidly couples the first well with the first opening and a second microconduit fluidly couples the second well with the second opening. An overlay is sealed to the outlet surface and penetrated by a deposition channel that is situated to communicate carrier fluid among the first opening, the second opening, and the deposition surface when the overlay is pressed against the deposition surface.
45 Citations
20 Claims
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1. A method of depositing at least one substance from a carrier fluid on a deposition surface, the method comprising:
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a) loading a carrier fluid having at least one substance into a first well of a spotter apparatus, wherein the spotter apparatus comprises; i) a loading surface including the first well and a second well; ii) an outlet surface, different than the loading surface, including a first opening and a second opening; iii) a first microconduit fluidly coupling the first well with the first opening; iv) a second microconduit fluidly coupling the second well with the second opening; and v) an overlay positioned on the outlet surface, wherein the overlay comprises a deposition channel that fully penetrates through the overlay, wherein the deposition channel is situated to communicate carrier fluid among the first opening, the second opening, and a deposition surface when the overlay is pressed against the deposition surface, b) pressing the overlay against the deposition surface to form a seal between the overlay and deposition surface, and c) flowing the carrier fluid in series from the first well, through the first microconduit to the deposition channel, through the second microconduit and into the second well, wherein the deposition channel directs at least one substance from the carrier fluid onto the deposition surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification