Biofilm inhibiting compositions enhancing weight gain in livestock

• US 10,301,339 B2
• Filed: 09/12/2018
• Issued: 05/28/2019
• Est. Priority Date: 08/11/2015
• Status: Active Grant

• Current Assignee: Akeso Biomedical, Inc.
• Original Assignee: Akeso Biomedical, Inc.
• Inventors: Ala'Aldeen, Dlawer, Mahdavi, Jafar, Soultanas, Panos
• Primary Examiner(s): Kosack, Joseph R
• Application Number:
  US16/129,611
• Publication Number:
  US 20190010180A1
• Time in Patent Office: 258 Days
• CPC Class Codes: A01N 37/10 Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
• CPC Class Codes: A01N 37/36 containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• CPC Class Codes: A01N 37/44 containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
• CPC Class Codes: A01N 59/16 Heavy metals; Compounds thereof
• CPC Class Codes: A23K 20/195 Antibiotics
• CPC Class Codes: A23K 20/30 Oligoelements
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/30 for swines
• CPC Class Codes: A23K 50/60 for weanlings
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23L 33/127 Antibiotics
• CPC Class Codes: A23L 33/16 Inorganic salts, minerals or trace elements
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 31/197 the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid carnitine A61K31/205
• CPC Class Codes: A61K 31/198 Alpha-amino acids, e.g. alanine or edetic acid [EDTA] betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00
• CPC Class Codes: A61K 31/295 Iron group metal compounds
• CPC Class Codes: A61K 31/7036 having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61L 12/08 using chemical substances
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: A61L 2/16 : using chemical substances
• CPC Class Codes: A61L 2/18 : Liquid substances or solutions comprising solids or dissolved gases
• CPC Class Codes: A61L 2/22 : Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
• CPC Class Codes: A61L 2202/24 : Medical instruments, e.g. endoscopes, catheters, sharps
• CPC Class Codes: A61P 31/00 : Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: C07F 15/025 : without a metal-carbon linkage
• CPC Class Codes: C09D 5/14 : Paints containing biocides, e.g. fungicides, insecticides or pesticides C09D5/16 takes precedence
• CPC Class Codes: G02B 1/043 : Contact lenses
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A method of enhancing the growth of an animal, as well as treating or preventing antimicrobial infections is provided. The method includes causing the animal to ingest or absorb an effective amount of one or more Fe III complex compounds, including but not limited to Fe III complexes comprising ligands bound to the iron center such as amino acids or α-hydroxy acids. The compounds are also useful for inhibiting, reducing, or preventing biofilm formation or buildup on a surface; the treatment of, inhibition of growth of, and inhibition of colonization by, bacteria, both in biological and non-biological environments; disinfecting surfaces, potentiating the effects of antibiotics and other anti-microbial agents, and increasing the sensitivity of bacteria and other microorganisms, to anti-microbial agents are also provided.

Claims

1. A method for reducing microbial infection or colonization in a mammal or bird, the method comprising administering to the mammal or bird an effective amount of a dietary product,

wherein the dietary product comprises one or more iron compounds, wherein the iron compound comprises an acid selected from the group consisting of alpha-hydroxyacid, amino acid, aminocarboxylic acid and diacid, bound to iron, and

wherein the iron compound is in a concentration range from 10 μ

M to 2 mM or 0.01 g/Kg of product to 20 g/Kg of product.

2. The method of claim 1, wherein the iron compound is a salt and/or a hydrate.

3. The method of claim 1, wherein the method reduces Campylobacter in the mammal or bird.

4. The method of claim 1, wherein the method reduces Salmonella in the mammal or bird.

5. The method of claim 1, wherein the method reduces Escherichia coli in the mammal or bird.

6. The method of claim 1, wherein the method reduces Clostridium in the mammal or bird.

7. The method of claim 6, wherein the method is used to reduce necrotic enteritis in the mammal or bird.

8. The method of claim 1, wherein the method improves feed conversion ratio, feed intake, weight gain and/or body weight.

9. The method of claim 1, wherein the compound comprises an acid selected from the group consisting of an amino acid, an alpha-hydroxyacid, EDTA and oxalic acid and wherein the iron compound is in a concentration range from 100 μ

M to 2 mM or 0.01 g/Kg of product to 20 g/Kg of product.

10. The method of claim 1, wherein the diacid is oxalic acid or malic acid.

11. The method of claim 1, wherein the method reduces Brachyspira in the mammal or bird.

12. The method of claim 1, wherein the aminocarboxylic acid is EDTA.

13. The method of claim 9, wherein the alpha-hydroxy acid is selected from the group consisting of citric acid, tartaric acid, lactic acid, glycolic acid, quinic acid, glycolic acid, isoleucic acid, valic acid, and mandelic acid.