High salinity tolerant microalgae strains, products derived thereform, and methods of producing the same

• US 10,301,589 B2
• Filed: 09/19/2016
• Issued: 05/28/2019
• Est. Priority Date: 10/19/2012
• Status: Active Grant

• Current Assignee: Khalifa University Of Science, Technology & Research
• Original Assignee: Khalifa University of Science and Technology
• Inventors: Al Harethi, Ahmed Husain, Hernandez, Hector Hugo
• Primary Examiner(s): Marx, Irene
• Application Number:
  US15/268,867
• Publication Number:
  US 20170096633A1
• Time in Patent Office: 981 Days
• Field of Search: None
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00
• CPC Class Codes: C12N 1/36 Adaptation or attenuation of cells
• CPC Class Codes: C12P 7/6409 Fatty acids
• CPC Class Codes: C12P 7/649 Biodiesel, i.e. fatty acid alkyl esters
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products.

Claims

1. A method of producing a high salinity tolerant microalgae strain comprising:

subjecting a microalgae cell in a medium to serially increased concentrations of a salt sufficient to produce the high salinity tolerant microalgae strain, wherein the microalgae strain is a green algae, a red algae or a diatom and the subjecting step comprises growing the microalgae cell in a medium having an initial salinity in a range from about 0 ppt to about 45 ppt and increasing the salt concentration to a salinity in a range from about 50 ppt to about 400 ppt and wherein the serially increased concentrations of the salt comprise a series of salt additions to the medium that incrementally increase the salinity of the medium to two or more salinity levels, thereby exposing the microalgae cell to the serially increased salt concentrations to produce the high salinity tolerant microalgae strain; and

recovering the high salinity tolerant microalgae cell, from the medium.

2. The method of claim 1, wherein the subjecting step comprises growing the microalgae cell at ambient air and/or ambient room temperature.

3. The method of claim 1, wherein the subjecting step comprises growing the microalgae cell using a natural and/or artificial light source.

4. The method of claim 1, wherein the series of salt additions comprises 2, 3, 4, 5, 6, 7, 8, 9 or 10 separate salt additions.

5. The method of claim 1, wherein the salinity is serially increased by about 5 ppt, 10 ppt, 20 ppt, 25 ppt, 30 ppt, 40 ppt, 50 ppt, or 75 ppt at each salt addition.

6. The method of claim 1, wherein the series of salt additions comprise two or more separate salt additions.

7. The method of claim 1, wherein the high salinity tolerant microalgae strain is produced in about 2 to about 14 days.

8. The method of claim 1, wherein the high salinity tolerant microalgae strain produces a secondary metabolite.

9. The method of claim 8, wherein the secondary metabolite is excreted by the high salinity tolerant microalgae strain.