Process of conducting high throughput testing high performance liquid chromatography
First Claim
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1. A process of conducting high throughput high performance liquid chromatography (HPLC) comprising:
- a) dropping containers of pre-weighed samples into plastic bottles;
b) adding solution to each set of container and bottle via a bottle top dispenser;
c) shaking each set of plastic bottle, container, and solution until sample is dissolved;
d) centrifuging each set of plastic bottle, container, and solution;
e) loading an aliquot of supernatant from the centrifuge step onto an HPLC column; and
f) running the column with a mobile phase.
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Abstract
The present invention utilizes a high throughput testing (HTT) method of high performance liquid chromatography (HPLC) to validate samples of pharmaceutical compositions. In one embodiment, improved sample preparation techniques comprise adding the entire vial of a sample to a wide mouth disposable bottle, adding diluent, shaking overnight, and centrifuging.
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Citations
16 Claims
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1. A process of conducting high throughput high performance liquid chromatography (HPLC) comprising:
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a) dropping containers of pre-weighed samples into plastic bottles; b) adding solution to each set of container and bottle via a bottle top dispenser; c) shaking each set of plastic bottle, container, and solution until sample is dissolved; d) centrifuging each set of plastic bottle, container, and solution; e) loading an aliquot of supernatant from the centrifuge step onto an HPLC column; and f) running the column with a mobile phase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification