Compositions, methods, and kits for quantifying target analytes in a sample
First Claim
1. A method of quantifying a target analyte by mass spectrometry, the method comprising:
- preparing a single sample by combining a first known quantity of a first calibrator, a second known quantity of a second calibrator, and a third known quantity of a third calibrator in a single sample comprising the target analyte,wherein the target analyte is an organic molecule comprising at least 3 carbon atoms,wherein the first calibrator, the second calibrator, and the third calibrator are each different stable isotope analogs of the target analyte,wherein the target analyte is unlabeled,wherein amount of the first calibrator, the second calibrator, and the third calibrator each differ exponentially with a base of 10 and an integer exponent;
generating a mass spectrometer signal from the single sample using a mass spectrometer comprising a first calibrator signal, comprising a second calibrator signal, comprising a third calibrator signal, and comprising a target analyte signal,wherein the first known quantity, the second known quantity, and the third known quantity are different, and wherein the first calibrator, the second calibrator, the third calibrator and the target analyte are each distinguishable in the single sample by mass spectrometry due to differences in their mass, fragmentation pattern or combinations thereof;
obtaining a calibration curve, wherein the calibration curve is obtained from the first calibrator signal, the second calibrator signal and third calibrator signal; and
quantifying the target analyte using the calibration curve and the target analyte signal.
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Abstract
A method of quantifying a target analyte by mass spectrometry includes obtaining a mass spectrometer signal comprising a first calibrator signal, comprising a second calibrator signal, and potentially comprising a target analyte signal from a single sample comprising a first known quantity of a first calibrator, comprising a second known quantity of a second calibrator, and potentially comprising a target analyte. The first known quantity and the second known quantity are different, and wherein the first calibrator, the second calibrator, and the target analyte are each distinguishable in the single sample by mass spectrometry. The method also includes quantifying the target analyte in the single sample using the first calibrator signal, the second calibrator signal, and the target analyte signal.
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Citations
18 Claims
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1. A method of quantifying a target analyte by mass spectrometry, the method comprising:
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preparing a single sample by combining a first known quantity of a first calibrator, a second known quantity of a second calibrator, and a third known quantity of a third calibrator in a single sample comprising the target analyte, wherein the target analyte is an organic molecule comprising at least 3 carbon atoms, wherein the first calibrator, the second calibrator, and the third calibrator are each different stable isotope analogs of the target analyte, wherein the target analyte is unlabeled, wherein amount of the first calibrator, the second calibrator, and the third calibrator each differ exponentially with a base of 10 and an integer exponent; generating a mass spectrometer signal from the single sample using a mass spectrometer comprising a first calibrator signal, comprising a second calibrator signal, comprising a third calibrator signal, and comprising a target analyte signal, wherein the first known quantity, the second known quantity, and the third known quantity are different, and wherein the first calibrator, the second calibrator, the third calibrator and the target analyte are each distinguishable in the single sample by mass spectrometry due to differences in their mass, fragmentation pattern or combinations thereof; obtaining a calibration curve, wherein the calibration curve is obtained from the first calibrator signal, the second calibrator signal and third calibrator signal; and quantifying the target analyte using the calibration curve and the target analyte signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification