Microfluidic detection system and a microfluidic cartridge

• US 10,307,754 B2
• Filed: 06/15/2015
• Issued: 06/04/2019
• Est. Priority Date: 06/18/2014
• Status: Active Grant

• Current Assignee: Zoetis Denmark ApS (Zoetis, Inc.)
• Original Assignee: Scandinavian Micro Biodevices ApS (Zoetis, Inc.)
• Inventors: Bau-Madsen, Niels Kristian, Nielsen, Lars Bue, Heller, Martin, Kring, Ole, Ordeig, Olga, Overby, Bent
• Primary Examiner(s): Siefke, Samuel P
• Application Number:
  US15/319,542
• Publication Number:
  US 20170203295A1
• Time in Patent Office: 1,450 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/02 Identification, exchange or storage of information
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/00811 consumable or exchangeable components other than sample carriers, e.g. detectors, flow cells
• CPC Class Codes: G01N 2035/00851 process control parameters
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/25 Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 2201/062 : LED's
• CPC Class Codes: G01N 2201/0627 : Use of several LED's for spectral resolution
• CPC Class Codes: G01N 27/403 : Cells and electrode assemblies
• CPC Class Codes: G01N 35/00029 : provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/00732 : Identification of carriers, materials or components in automatic analysers

Abstract

A microfluidic cartridge includes first and second sides and at least one flow channel and an inlet to the flow channel(s) for feeding a liquid sample, the flow channel(s) include a plurality of first optical detection sites. A detector assembly includes a slot for inserting the microfluidic cartridge and a first fixed light source with a beam path and an optical reader for reading out optical signals from at least one of the first optical detection site(s). When the microfluidic cartridge is inserted to a first predetermined position into the slot, one of the first optical detection sites of the microfluidic cartridge is positioned in the beam path of the first light source, and when the cartridge is inserted to a second predetermined position into the slot, another one of the first optical detection sites of the microfluidic cartridge is positioned in the beam path of the first light source.

Claims

1. A microfluidic detection system comprising a microfluidic cartridge and a detector assembly,

the microfluidic cartridge comprises a first and a second side and at least one flow channel and an inlet to the at least one flow channel for feeding a liquid sample, the at least one flow channel comprises a plurality of first optical detection sites, and

the detector assembly comprises a slot for inserting the microfluidic cartridge and a first fixed light source with a beam path and an optical reader for reading out optical signals from at least one of said first optical detection sites, said detector assembly and the microfluidic cartridge are constructed such that when the microfluidic cartridge is inserted to a first predetermined position into said slot, one of said first optical detection sites of the microfluidic cartridge is positioned in the beam path of the first light source, and when the cartridge is inserted to a second predetermined position into said slot, another one of the first optical detection sites of the microfluidic cartridge is positioned in the beam path of the first light source, wherein each of said first and said second predetermined positions of said microfluidic cartridge into said slot are determined by projecting flanges arranged on the microfluidic cartridge and cavities arranged on the detector assembly at selected positions or projecting flanges arranged on the detector assembly and cavities arranged on the microfluidic cartridge at the selected positions, which engage or snap into place to temporarily position the microfluidic cartridge in the detector assembly at one of the first and the second predetermined positions, said projecting flanges and cavities comprises a first set of projecting flanges and cavities which correspond to the first predetermined position and said projecting flanges and cavities comprises a second set of projecting flanges and cavities which correspond to the second predetermined position.

2. The microfluidic detection system of claim 1, wherein said first light source comprises a multicolor light emitting diode (LED) configured for emitting a plurality of different light beams having different wavelengths and a circuitry for switching said plurality of different light beams on and off.

3. The microfluidic detection system of claim 1, wherein the slot of the detector assembly and the microfluidic cartridge are constructed such that when said microfluidic cartridge is inserted to a predetermined position into said slot, one of the first optical detection sites of the microfluidic cartridge is positioned in the beam path of the first light source, the predetermined position of said microfluidic cartridge into said slot is determined by a click arrangement holding the microfluidic cartridge in a temporally fixed position.

4. The microfluidic detection system of claim 1, wherein the optical reader is arranged for reading out at least one of an absorption property, a reflection property or an emitting property from a liquid sample in at least one of said first detection sites when said cartridge is inserted into said slot of said detector assembly.

5. The microfluidic detection system of claim 1, wherein the optical reader is a digital imaging reader.

6. The microfluidic detection system of claim 1, wherein the optical reader comprises a charge-coupled device (CCD) reader.

7. The microfluidic detection system of claim 1, wherein the circuitry of said first light source is configured for switching said plurality of different light beams on and off independently of each other.

8. The microfluidic detection system of claim 1, wherein the detector assembly is programmed to switch the plurality of different light beams on and off such that only one of the different light beams is switched on at a time.

9. The microfluidic detection system of claim 1, wherein the plurality of different light beams comprise from 2 to 5 different light beams.

10. The microfluidic detection system of claim 1, wherein each of the plurality of different light beams independently of each other have a spectral width of up to about 50 nm.

11. The microfluidic detection system of claim 1, wherein said plurality of different light beams of said multicolor-LED are monochromatic light beams.

12. The microfluidic detection system of claim 1, wherein said plurality of different light beams of said multicolor-LED comprise at least one of a light beam having a center wavelength of about 575 nm to about 625 nm or a light beam having a center wavelength of about 425 nm to about 475 nm.

13. The microfluidic detection system of claim 1, wherein said plurality of different light beams of said multicolor-LED comprise at least three monochromatic light beams selected from red, orange, yellow, green or blue light beams.

14. The microfluidic detection system of claim 1, wherein said at least one flow channel of said microfluidic cartridge comprises a plurality of additional optical detection sites, and the detector assembly comprises at a plurality of additional fixed light source with respective beam paths, the slot of the detector is shaped such that when said microfluidic cartridge is inserted into said slot, the plurality of additional optical detection sites of the microfluidic cartridge are positioned in respective beam paths of the plurality of additional light sources, each of said plurality of additional light sources comprises a multicolor light emitting diode (LED) configured for emitting a plurality of different light beams having different wavelengths and a circuitry for switching said plurality of different light beams on and off, said optical reader is configured for reading out optical signals from said additional optical detection sites and said detector assembly comprises at least one additional optical reader configured for reading out optical signals from said additional optical detection sites.

15. The microfluidic detection system of claim 1, wherein said detector assembly comprises a light tunnel for one or more of the fixed light sources to prevent the beams from the respective fixed light sources to transmit light to two or more detection sites simultaneously.

16. The microfluidic detection system of claim 1, wherein said at least one flow channel of said microfluidic cartridge comprises a plurality of detection cites for performing a plurality of different assays.

17. The microfluidic detection system of claim 16, wherein the plurality of detection sites comprise at least one electrical detection site, said electrical detection site comprises electrodes arranged for performing an electrochemical detection at the electrical detection site, said electrodes comprise electrical wires connected to microfluidic cartridge connection pads, the detector assembly comprises at least one electrical reader for reading out electrical signals out from the electrical detection sites.

18. The microfluidic detection system of claim 17, wherein the electrical reader comprises a voltmeter electrically connected to voltmeter connection pads arranged in the slit such that the microfluidic cartridge connection pads are in electrical connection with said voltmeter connection pads when said microfluidic cartridge is inserted into said slot.

19. The microfluidic detection system of claim 1, wherein said detector assembly comprises at least one output interface and a processor, said microfluidic cartridge comprises a machine readable code comprising instructions about assays to be performed using the cartridge and said detector assembly comprises a code reader for reading the machine readable code and feeding the instructions about the assays to be performed to the processor, wherein the processor is programmed to control at least one of the readers and the output interface at least partly based on instructions obtained from the machine readable code, preferably said at least one reader is at least one of the optical reader and the electrical reader.