×

Anti-GP73 monoclonal antibodies and methods of obtaining the same

  • US 10,308,709 B2
  • Filed: 10/11/2016
  • Issued: 06/04/2019
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
Patent Images

1. A method for determining GP73 concentration in a test sample, the method comprising:

  • (a) contacting the test sample with at least one capture antibody comprising (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;

    18, a CDR2 comprising the amino acid sequence of SEQ ID NO;

    19, and a CDR3 comprising the amino acid sequence of SEQ ID NO;

    20, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;

    22, a CDR2 comprising the amino acid sequence of SEQ ID NO;

    23, and a CDR3 comprising the amino acid sequence of SEQ ID NO;

    24, which binds to an epitope on GP73 comprising the amino acid sequence of SEQ ID NO;

    102 to form a capture antibody-GP73 antigen complex;

    (b) contacting the capture antibody-GP73 antigen complex with at least one detection antibody comprising a detectable label, wherein the detection antibody comprises (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;

    26, a CDR2 comprising the amino acid sequence of SEQ ID NO;

    27, and a CDR3 comprising the amino acid sequence of SEQ ID NO;

    28, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;

    30, a CDR2 comprising the amino acid sequence of SEQ ID NO;

    31, and a CDR3 comprising the amino acid sequence of SEQ ID NO;

    32 which binds to an epitope on GP73 that is not bound by the capture antibody and forms a capture antibody-GP73 antigen-detection antibody complex; and

    (c) determining the GP73 concentration in the test sample based on the signal generated by the detectable label in the capture antibody-GP73 antigen-detection antibody complex formed in (b).

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×