Anti-GP73 monoclonal antibodies and methods of obtaining the same
First Claim
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1. A method for determining GP73 concentration in a test sample, the method comprising:
- (a) contacting the test sample with at least one capture antibody comprising (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
18, a CDR2 comprising the amino acid sequence of SEQ ID NO;
19, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
20, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
22, a CDR2 comprising the amino acid sequence of SEQ ID NO;
23, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
24, which binds to an epitope on GP73 comprising the amino acid sequence of SEQ ID NO;
102 to form a capture antibody-GP73 antigen complex;
(b) contacting the capture antibody-GP73 antigen complex with at least one detection antibody comprising a detectable label, wherein the detection antibody comprises (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
26, a CDR2 comprising the amino acid sequence of SEQ ID NO;
27, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
28, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
30, a CDR2 comprising the amino acid sequence of SEQ ID NO;
31, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
32 which binds to an epitope on GP73 that is not bound by the capture antibody and forms a capture antibody-GP73 antigen-detection antibody complex; and
(c) determining the GP73 concentration in the test sample based on the signal generated by the detectable label in the capture antibody-GP73 antigen-detection antibody complex formed in (b).
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Abstract
Disclosed herein are antibodies and methods of using said antibodies to detect Golgi protein 73 (GP73) and fucosylated GP73 in a sample.
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5 Claims
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1. A method for determining GP73 concentration in a test sample, the method comprising:
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(a) contacting the test sample with at least one capture antibody comprising (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
18, a CDR2 comprising the amino acid sequence of SEQ ID NO;
19, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
20, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
22, a CDR2 comprising the amino acid sequence of SEQ ID NO;
23, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
24, which binds to an epitope on GP73 comprising the amino acid sequence of SEQ ID NO;
102 to form a capture antibody-GP73 antigen complex;(b) contacting the capture antibody-GP73 antigen complex with at least one detection antibody comprising a detectable label, wherein the detection antibody comprises (i) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
26, a CDR2 comprising the amino acid sequence of SEQ ID NO;
27, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
28, and (ii) a variable light chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO;
30, a CDR2 comprising the amino acid sequence of SEQ ID NO;
31, and a CDR3 comprising the amino acid sequence of SEQ ID NO;
32 which binds to an epitope on GP73 that is not bound by the capture antibody and forms a capture antibody-GP73 antigen-detection antibody complex; and(c) determining the GP73 concentration in the test sample based on the signal generated by the detectable label in the capture antibody-GP73 antigen-detection antibody complex formed in (b). - View Dependent Claims (2, 3, 4, 5)
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Specification