Oscillating amplification reaction for nucleic acids
First Claim
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1. A method for amplifying a target nucleic acid sequence contained in a sample, the method comprising:
- contacting the sample with an amplification reaction mixture comprising a primer complementary to the target nucleic acid sequence;
oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20°
C. during a plurality of temperature cycles; and
amplifying the target nucleic acid sequence.
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Abstract
One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
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Citations
47 Claims
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1. A method for amplifying a target nucleic acid sequence contained in a sample, the method comprising:
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contacting the sample with an amplification reaction mixture comprising a primer complementary to the target nucleic acid sequence; oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20°
C. during a plurality of temperature cycles; andamplifying the target nucleic acid sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification