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Oscillating amplification reaction for nucleic acids

  • US 10,316,358 B2
  • Filed: 08/25/2016
  • Issued: 06/11/2019
  • Est. Priority Date: 04/20/2011
  • Status: Active Grant
First Claim
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1. A method for amplifying a target nucleic acid sequence contained in a sample, the method comprising:

  • contacting the sample with an amplification reaction mixture comprising a primer complementary to the target nucleic acid sequence;

    oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20°

    C. during a plurality of temperature cycles; and

    amplifying the target nucleic acid sequence.

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