×

Formation and calibration of nanopore sequencing cells

  • US 10,317,392 B2
  • Filed: 06/23/2017
  • Issued: 06/11/2019
  • Est. Priority Date: 06/23/2016
  • Status: Active Grant
First Claim
Patent Images

1. A method of using a sequencing cell, the method comprising:

  • obtaining a plurality of measured signal values for the sequencing cell having a voltage applied across the sequencing cell, the sequencing cell including a nucleic acid, wherein the applied voltage includes an alternating signal having a first portion and a second portion relative to a reference voltage;

    determining a first set of one or more signal values measured during the first portion of the alternating signal;

    determining a second set of one or more signal values measured during the second portion of the alternating signal, wherein the first set of one or more signal values and second set of one or more signal values are determined with no molecule in a nanopore of the sequencing cell;

    determining a normalization factor based on the second set of one or more signal values;

    determining a third set of one or more signal values measured during the first portion of the alternating signal when a tag molecule is threaded in the nanopore of the sequencing cell, the tag molecule corresponding to a particular nucleotide; and

    normalizing the third set of one or more signal values using the normalization factor to obtain one or more normalized signal values.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×