Apparatus and method for preparing cosmeceutical ingredients containing epi-dermal delivery mechanisms

• US 10,322,074 B2
• Filed: 03/15/2017
• Issued: 06/18/2019
• Est. Priority Date: 12/16/2015
• Status: Active Grant

• Current Assignee: PPP&C Inc.
• Original Assignee: PPP&C Inc.
• Inventors: Richard, David A.
• Primary Examiner(s): Maewall, Snigdha
• Application Number:
  US15/460,105
• Publication Number:
  US 20170181940A1
• Time in Patent Office: 825 Days
• CPC Class Codes: A61K 2800/70 Biological properties of the composition as a whole
• CPC Class Codes: A61K 2800/805 Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• CPC Class Codes: A61K 8/06 Emulsions
• CPC Class Codes: A61K 8/14 Liposomes; Vesicles
• CPC Class Codes: A61K 8/416 Quaternary ammonium compounds A61K8/35 takes precedence
• CPC Class Codes: A61K 8/553 Phospholipids, e.g. lecithin
• CPC Class Codes: A61K 8/63 Steroids; Derivatives thereof
• CPC Class Codes: A61K 8/735 Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
• CPC Class Codes: A61K 8/9789 Magnoliopsida [dicotyledons]
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

The skin serves as a barrier that protects the body from the external environment and prevents water loss. This barrier function also prevents most hydrophilic or hydrophobic and large molecular weight ingredients (>500 kDa) from penetrating intact skin. Until recently, methods to increase stratum corneum permeability were generally not effective enough to make the stratum corneum so permeable that the barrier posed by the viable epidermis mattered. However, that has now changed with the development of the present embodiment'"'"'s physical methods and highly optimized chemical formulations, such that we revisited the permeability of the full epidermis with the example embodiment'"'"'s constructs and not focus only on the stratum corneum. This example embodiment therefore tests the hypothesis that the viable epidermis offers a significant permeability barrier to both small molecules and macromolecules that becomes the rate limiting step.

Claims

1. A method for the construction of cosmeceutically bioactive compositions including elastic niosome vesicle epi-dermal delivery vehicles without use of supercritical CO₂ and in a continuously operating process, the method comprising:

forming a liposome solution or mixture of cosmeceutically benevolent phospholipids, the solution being hydrophobic or hydrophilic, the solution including an ingredient from the group consisting of a natural ingredient and a synthetic ingredient, the solution having an aqueous phase;

removing any constituent attributes of water-insolubility from the solution, while operating under conditions to preserve activity of labile biomolecules;

loading the liposome solution and desired hydrophobic bio-actives into an organic solvent residing in a pressure reactor, the pressure reactor having been previously driven to a working temperature less than 31.1°

C.;

pressurizing the reactor with compressed CO₂ until reaching a working pressure less than 73.8 bar to produce a resulting CO₂-expanded solution, the reactor maintaining a pressure and temperature to prevent the CO₂ from going supercritical;

depressurizing the resulting CO₂-expanded solution over an aqueous phase to form vesicular conjugates, the resulting solution containing water soluble cationic or non-ionic surfactants and hydrophilic or hydrophobic bio-actives; and

forming nano and macro carriers as either unimolecular or multi-molecular carriers of beneficial cosmeceutical ingredients.

2. The method of claim 1 including using a neutral lipid species, selected from the group consisting of phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, and cholesterol.

3. The method of claim 1 including forming nano and macro carriers as unimolecular carriers, having dendrimers, carbon nanotubes, polymer-conjugate drug/protein, or multimolecular carriers.

4. The method of claim 1 wherein the nano and macro carriers are based on molecular self-assemblies having nanoshells, vesicles, micelles, elastic vesicles, liquid crystal phases, and Langmuir monolayers by surfactant molecules in aggregate structures having a translation aggregate, a glide aggregate, and a screw aggregate.

5. The method of claim 1 wherein the nano and macro carriers have an effective diameter of between 30 and 1500 nanometers, between 150 to 4000 nanometers, between 50 to 380 nanometers or 1 to 35 microns.

6. The method of claim 1 wherein the nano and macro carriers have a polydispersity of between 0.001 to 1, between 0.01 to 0.9, or between 0.1 to 0.6.

7. The method of claim 1 wherein the nano and macro carriers have an ingredient lipid content % w/w of between 25 to 200, 50 to 100, or 60 to 90.

8. The method of claim 1 wherein the nano and macro carriers have residual solvent levels % v/v between 0.01 to 10, 0.1 to 6, or 0.3 to 5.

9. The method of claim 1 including forming a cosmeceutical agent preparation, incorporated into particles having a diameter of 0.5 to 100 microns, the preparation comprising a core, the core comprising at least one member selected from the group consisting of a hydrophilic material and a hydrophobic material and at least first and second layers, each member comprising at least one member selected from the group consisting of hydrophilic material and hydrophobic material wherein an interface between the core and the first layer and between adjacent layers is an interface between hydrophobic and hydrophilic material.

10. The method of claim 1 including introducing a cosmeceutically active agent into a cell of a plant or animal, the method further including:

preparing lipid vesicles comprising a cationic, anionic, or non-ionic lipid and containing the cosmeceutically active agent; and

contacting the cell with the elastic lipid vesicles whereby the cosmeceutically active agent is taken up into the cell.

11. The method of claim 1 including introducing a cosmeceutically active agent into a cell of a plant or animal, the method further including:

preparing lipid vesicles comprising a cationic, anionic, or non-ionic lipid and containing the cosmeceutically active agent; and

contacting the cell with a bioactive agent in the presence of the lipid vesicles, whereby the bioactive agent is taken up into the cell.

12. The method of claim 1 wherein a final arrangement of the liposome solution or mixture is governed by the nature of the initial components and the methodology used in their preparation.

13. The method of claim 9 wherein at least one of the layers is an emulsion.

14. The method of claim 9 wherein the core comprises an emulsion of a hydrophobic material and a hydrophilic material as an oil-in-water emulsion.

15. The method of claim 9 wherein the core comprises an emulsion of a hydrophilic material and a hydrophobic material as a water-in-oil emulsion.

16. The method of claim 1 including forming a combination of the liposome solution or mixture, a hydrophobic or hydrophilic cosmeceutical ingredient, and an aqueous phase and a low-pressure fluid, the combination being decompressed to separate the low pressure, critical fluid, from the phospholipid and aqueous medium, to form one or more liposomes or elastic vesicles, the rate of depressurization influences the size of the liposomes formed.

17. The method of claim 1 including loading the liposome solution and desired hydrophobic bio-actives in an organic solvent into the high-pressure reactor.

18. The method of claim 1 wherein the nano and macro carriers enable the incorporation of cosmeceutical essential oils into carrier materials by facilitating the diffusion of the essential oil due to the swelling and opening of the pores of carrier material particles.

19. The method of claim 1 including forming unilamellar liposome or niosome vesicles, the size of the liposome or niosome being determined by the rate of decompression.

20. The method of claim 1 wherein during the depressurization step, the resulting solution experiences an abrupt and extremely homogenous temperature decrease produced by CO₂ evaporation from the resulting solution, thereby producing homogenous vesicles regarding size, lamellarity and morphology.

21. The method of claim 1 wherein the vesicular conjugates allow the physicochemical properties of ingredient molecules, of a molecular weight of from 1 to 700 kDa, in a liposomal system to be changed, which facilitates crossing of the stratum corneum barrier into the epi-dermis.

22. The method of claim 1 wherein the phospholipids are composed of a bilayer of amphipathic molecules, the molecules arranged in two concentric circles, such that the hydrophilic heads of the outer layer exposed to the outer environment, and the hydrophilic heads of the inner layer make the inner hydrophilic core, hydrophobic tails being tucked between the two layers.

23. The method of claim 1 wherein the elastic niosome vesicles are microscopic vesicles having single or multiple phospholipid bilayers, which can entrap hydrophobic or hydrophilic compounds within their aqueous cores.

24. The method of claim 1 wherein the phospholipids are naturally derived from phospholipids obtained from plant or animal sources, the phospholipid being salted or desalted, hydrogenated or partially hydrogenated or natural, semi-synthetic, or synthetic.

25. The method of claim 9 wherein the particles being unilamellar liposomal spherical particles ranging in size from 35 to 200 nm or 35-100 nm.

26. The method of claim 1 wherein the molar ratio of the water soluble cationic or non-ionic surfactants and hydrophilic or hydrophobic bio-actives to neutral lipid species is from 9/1 to 1/9.

27. The method of claim 26 wherein the molar ratio is 5/5.

28. The method of claim 1 wherein a cationic, anionic or non-ionic lipid has a structure with a pharmacologically effective amount of therapeutic agent.

29. The method of claim 1 including using the composition for topical use, and having a pharmacologically effective amount of a therapeutic agent in a cosmeceutically acceptable vehicle.

30. The method of claim 1 including administering a cosmeceutical preparation comprising a cationic, anionic, or non-ionic lipid having a cosmeceutically effective amount of an active agent specific for the treatment, and permitting the cosmeceutical agent to be incorporated into at least one cell of the vertebrate, whereby an appearance is effectively treated.

31. The method of claim 1 including administering a topical application of vesicles having single or multiple phospholipid preparation to the skin surface.

32. The method of claim 10 wherein the active agent is a cosmeceutical.