Fluidic flow cytometry devices and particle sensing based on signal-encoding

• US 10,324,018 B2
• Filed: 10/02/2017
• Issued: 06/18/2019
• Est. Priority Date: 03/10/2009
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Lo, Yu-Hwa, Chen, Chun Hao Randy, Cho, Sung Hwan, Tsai, Shang-Feng
• Primary Examiner(s): Stock, Jr., Gordon J
• Application Number:
  US15/722,550
• Publication Number:
  US 20180202916A1
• Time in Patent Office: 624 Days
• Field of Search: 356 39- 42, 356335-343, 356402-425, 356432, 356444
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/1425 using an analyser being characterised by its control arrangement
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1415 Control of particle position
• CPC Class Codes: G01N 2015/1447 Spatial selection
• CPC Class Codes: G01N 2015/145 by pattern of light, e.g. fringe pattern

Abstract

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements.

Claims

1. A method, comprising:

receiving a particle detection signal at a detector, the particle detection signal associated with optical characteristics of a particle in a first channel;

identifying, based on the particle detection signal, a sorting channel among a plurality of second channels coupled to the first channel for sorting the particle into the sorting channel based on the optical characteristics of the particle;

transmitting a sorting signal for sorting the particle from the first channel into the identified sorting channel;

receiving a verification signal at the detector, the verification signal associated with the presence of a detected particle in the sorting channel, the verification signal being an optical signal; and

verifying the sorting of the detected particle from the first channel into the sorting channel based on the verification signal.

2. The method of claim 1, wherein the particle is a first particle and the sorting signal is a first sorting signal, the method further comprising transmitting a second sorting signal for sorting a second particle, the second sorting signal based on the particle detection signal and on the verification signal.

3. The method of claim 1, when the verifying is indicative of an error in the sorting of the particle, further comprising generating an alert indicative of the error.

4. The method of claim 1, when the verifying is indicative of an error in the sorting of the particle, further comprising generating a signal to stop flow in the first channel.

5. The method of claim 1, when the verifying is indicative of an error in the sorting of the particle, further comprising generating a signal to stop generation of additional sorting signals.

6. The method of claim 1, further comprising generating, at the detector, a detector signal representative of the particle detection signal and of the verification signal.

7. An apparatus, comprising:

a first channel configured to receive a fluid flow including one or more particles;

a plurality of second channels downstream of the first channel;

a detector configured to receive a particle detection signal, the particle detection signal associated with optical characteristics of a particle in the first channel; and

a processor communicably coupled to the detector and configured to;

identify a sorting channel of the plurality of second channels based on the particle detection signal for sorting the particle into the sorting channel based on the optical characteristics of the particle, and

transmit a sorting signal for sorting the particle from the first channel into the sorting channel,

the detector further configured to receive a verification signal, the verification signal associated with the presence of a detected particle in the sorting channel, the verification signal being an optical signal,

the processor further configured to verify the sorting of the detected particle from the first channel into the sorting channel based on the verification signal.

8. The apparatus of claim 7, wherein the particle is a first particle and the sorting signal is a first sorting signal, the processor further configured to transmit a second sorting signal for sorting a second particle, the second sorting signal based on the particle detection signal and on the verification signal.

9. The apparatus of claim 7, wherein, when a verification by the processor is indicative of an error in the sorting of the particle, the processor is further configured to generate an alert indicative of the error.

10. The apparatus of claim 7, wherein, when a verification by the processor is indicative of an error in the sorting of the particle, the processor is further configured to generate a signal to stop fluid flow in the first channel.

11. The apparatus d of claim 7, wherein, when a verification by the processor is indicative of an error in the sorting of the particle, the processor is further configured to generate a signal to stop generation of additional sorting signals.

12. The apparatus of claim 7, the detector further configured to generate a detector signal representative of the particle detection signal and of the verification signal.

13. The apparatus of claim 7, further including an actuator configured to receive the sorting signal and to generate a pressure pulse in the first channel for sorting the particle from the first channel into the sorting channel.

14. The apparatus of claim 7, further including a light source configured to generate a light signal to illuminate the particle in the first channel, the particle detection signal being generated in response to the light signal.