Fluidic optical cartridge

• US 10,324,020 B2
• Filed: 12/23/2013
• Issued: 06/18/2019
• Est. Priority Date: 12/23/2013
• Status: Active Grant

• Current Assignee: Xerox Corporation (Xerox Holdings Corp.)
• Original Assignee: Palo Alto Research Center, Inc. (Xerox Holdings Corp.)
• Inventors: Johnson, David M., Johnson, Noble M., Recht, Michael I., Martini, Joerg, Curley, Tim, Kiesel, Peter, Sheridan, Martin
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US14/139,317
• Publication Number:
  US 20150177118A1
• Time in Patent Office: 2,003 Days
• Field of Search: None
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/145 by pattern of light, e.g. fringe pattern

Abstract

Embodiments are directed to an apparatus that includes a fluidic structure and optical components. The fluidic structure includes a transparent channel through which objects in an analyte fluid can travel along respective paths during operation of the apparatus. The optical components are configured to provide measurement light to the objects traveling through the transparent channel. The fluidic structure is configured to reversibly engage with a host structure. The host structure includes a source of the measurement light and electronics to receive and process output light emanating from the objects traveling in the channel. The fluidic structure makes an air-tight seal when engaged with the host structure.

Claims

1. An apparatus configured to engage with a host structure, the apparatus comprising:

a base plate;

a fluidic structure that includes;

a channel having a cross-section dimension through which objects can travel along respective paths during operation of the apparatus, the channel having at least one transparent wall;

a first end portion affixed to the base plate, and comprising an inlet coupled to a first end of the channel, wherein the inlet is configured to engage a pipettor tip;

a second end portion coupled to a second end of the channel, the second end of the channel positioned opposite the first end of the channel, the second end portion having a cross-section dimension larger than the cross-section dimension of the channel; and

a flange coupled to the second end portion and configured to reversibly engage with the host structure;

at least one optical component affixed to the base plate and disposed in a fixed spatial relationship with the fluidic structure, and disposed in a path of measurement light, the measurement light passing from the optical component into the fluidic structure through the transparent wall to the objects traveling through the channel, output light emanating from the objects traveling in the channel in response to the measurement light and passing out of the fluidic structure through the transparent wall; and

at least one optical filter assembly positioned directly in contact with the at least one transparent wall.

2. An apparatus according to claim 1, wherein the optical component comprises a lens that redirects the measurement light toward the transparent wall.

3. An apparatus according to claim 1, further comprising at least one optical filter assembly disposed in a path of one or more of the measurement light and the output light and affixed to the base plate, the at least one optical filter assembly configured to optically filter one or more of the measurement light and the output light.

4. An apparatus according to claim 3, wherein the at least one filter assembly is disposed opposing the transparent wall of the channel.

5. An apparatus according to claim 1, wherein the channel comprises the at least one optical filter assembly.

6. An apparatus according to claim 1, wherein the measurement light comes from a laser, a laser diode, a light-emitting diode, a superluminescent diode, a diode-pumped solid state laser, a frequency-doubled laser, a frequency-tripled laser, or a frequency-quadrupled laser.

7. An apparatus according to claim 1, wherein the at least one optical component comprises two optical components, each of the optical components being configured to receive measurement light from a different light source than the other optical component.

8. An apparatus according to claim 1, comprising two or more optical components.

9. An apparatus according to claim 1, wherein the at least one filter assembly comprises a spatial filter.

10. An apparatus according to claim 3, wherein the fluidic structure and the at least one optical filter assembly are in a fixed spatial relationship to each other.

11. An apparatus according to claim 1, wherein the second end portion comprises a taper.

12. An apparatus according to claim 1, wherein the flange is configured to engage and interlock with an interlock tab of the host structure.

13. An apparatus according to claim 1, wherein the apparatus comprises polycarbonate, poly(methyl methacrylate), polypropylene, or polyethylene.

14. An apparatus according to claim 1, wherein the optical component comprises a lens array that redirects the measurement light toward the transparent wall.

15. An apparatus according to claim 1, wherein the optical component comprises a microlens array that redirects the measurement light toward the transparent wall.

16. An apparatus according to claim 1, wherein the optical component comprises a mirror that redirects the measurement light toward the transparent wall.

17. An apparatus according to claim 1, wherein the optical component comprises a micromirror array that redirects the measurement light toward the transparent wall.