System and methods for bilirubin analysis
First Claim
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1. A method for measuring bilirubin levels in a subject, the method comprising:
- providing a fluid sample to be measured from the subject to a microfluidic device, wherein the sample comprises bilirubin bound to albumin;
contacting the fluid sample and a release agent in the form of a dry solid, the release agent configured to release the bound bilirubin from the albumin;
measuring electrochemical data of the sample using an electrochemical cell, wherein the electrochemical cell comprises screen printed electrodes; and
determining a total serum bilirubin concentration of the sample using the electrochemical data.
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Abstract
A method for measuring bilirubin levels in a subject. The method can include the steps of providing a sample to be measured from the subject, wherein the sample comprises bilirubin bound to albumin; adding a release agent to the sample, the release agent configured to release the bound bilirubin from the albumin; measuring electrochemical data of the sample using an electrochemical cell; and determining a total serum bilirubin concentration of the sample using the electrochemical data. This method is capable of providing a simpler, faster, and more robust measurement when compared to traditional bilirubin assay methods.
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Citations
20 Claims
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1. A method for measuring bilirubin levels in a subject, the method comprising:
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providing a fluid sample to be measured from the subject to a microfluidic device, wherein the sample comprises bilirubin bound to albumin; contacting the fluid sample and a release agent in the form of a dry solid, the release agent configured to release the bound bilirubin from the albumin; measuring electrochemical data of the sample using an electrochemical cell, wherein the electrochemical cell comprises screen printed electrodes; and determining a total serum bilirubin concentration of the sample using the electrochemical data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 19)
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8. A method for diagnosing a subject based on electrochemical detection of analytes, the method comprising:
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providing a blood sample to be measured from the subject to a microfluidic device, wherein the sample comprises both unbound bilirubin and bilirubin bound to albumin; contacting the blood sample and a release agent in the form of a dry solid, the release agent configured to release the bound bilirubin from the albumin; measuring electrochemical data of the sample using an electrochemical cell, wherein the electrochemical cell comprises screen printed electrodes; determining concentrations of conjugated bilirubin, unconjugated bilirubin, total serum bilirubin, and albumin using the electrochemical data; and making a diagnosis of an illness for the subject based on the determined concentrations of conjugated bilirubin, unconjugated bilirubin, total serum bilirubin, and albumin. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20)
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Specification