Continuous cell programming devices
First Claim
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1. A method of continuous in situ dendritic cell programming, comprising administering to a subject a device comprisinga scaffold polymer selected from the group consisting of poly-lactide-co-glycolide (PLG), alginate, xantham gum, gellan, or emulsan;
- a tumor antigen;
a recruitment composition comprising a cytokine, which comprises a granulocyte macrophage colony stimulating factor (GM-CSF) that recruits one or more dendritic cells to temporarily reside within said device; and
a positively charged polyethylenimine (PEI)-condensed toll-like receptor-9 (TLR-9)-activating CpG-ODN which is retained in or on said scaffold polymer,wherein said device attracts a dendritic cell and enhances uptake of said CpG-ODN into said dendritic cell, thereby continuously stimulating said dendritic cell to induce an immune response, and wherein said PEI condensed CpG-ODN overcomes the trapping effect of the cytokine on said dendritic cell, thereby inducing said dendritic cell to migrate away from said device.
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Abstract
The present invention comprises compositions, methods and devices for creating an infection-mimicking environment within a polymer scaffold to stimulate antigen-specific dendritic cell activation. Devices of the present invention are used to provide protective immunity to subjects against infection and cancer.
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8 Claims
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1. A method of continuous in situ dendritic cell programming, comprising administering to a subject a device comprising
a scaffold polymer selected from the group consisting of poly-lactide-co-glycolide (PLG), alginate, xantham gum, gellan, or emulsan; -
a tumor antigen; a recruitment composition comprising a cytokine, which comprises a granulocyte macrophage colony stimulating factor (GM-CSF) that recruits one or more dendritic cells to temporarily reside within said device; and a positively charged polyethylenimine (PEI)-condensed toll-like receptor-9 (TLR-9)-activating CpG-ODN which is retained in or on said scaffold polymer, wherein said device attracts a dendritic cell and enhances uptake of said CpG-ODN into said dendritic cell, thereby continuously stimulating said dendritic cell to induce an immune response, and wherein said PEI condensed CpG-ODN overcomes the trapping effect of the cytokine on said dendritic cell, thereby inducing said dendritic cell to migrate away from said device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification