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Continuous cell programming devices

  • US 10,328,133 B2
  • Filed: 02/13/2009
  • Issued: 06/25/2019
  • Est. Priority Date: 02/13/2008
  • Status: Active Grant
First Claim
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1. A method of continuous in situ dendritic cell programming, comprising administering to a subject a device comprisinga scaffold polymer selected from the group consisting of poly-lactide-co-glycolide (PLG), alginate, xantham gum, gellan, or emulsan;

  • a tumor antigen;

    a recruitment composition comprising a cytokine, which comprises a granulocyte macrophage colony stimulating factor (GM-CSF) that recruits one or more dendritic cells to temporarily reside within said device; and

    a positively charged polyethylenimine (PEI)-condensed toll-like receptor-9 (TLR-9)-activating CpG-ODN which is retained in or on said scaffold polymer,wherein said device attracts a dendritic cell and enhances uptake of said CpG-ODN into said dendritic cell, thereby continuously stimulating said dendritic cell to induce an immune response, and wherein said PEI condensed CpG-ODN overcomes the trapping effect of the cytokine on said dendritic cell, thereby inducing said dendritic cell to migrate away from said device.

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