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Methods and compositions for incorporating nucleotides

  • US 10,329,611 B2
  • Filed: 03/05/2018
  • Issued: 06/25/2019
  • Est. Priority Date: 03/19/2008
  • Status: Active Grant
First Claim
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1. A method for determining an identity of a nucleotide at a position, comprising:

  • a) providing a device which measures color in more than one detector channel, nucleic acid, polymerase, and a mixture of labeled and non-labeled reversible terminators, said labeled reversible terminators comprising a label linked to a nucleotide analogue, said label producing color, said color detected in two channels;

    b) incorporating, with said polymerase, a first nucleotide analogue from said mixture into said nucleic acid at a position;

    c) detecting the label of said incorporated first nucleotide analogue in two channels with said device; and

    d) determining the identity of the incorporated nucleotide at said position.

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