Mass produced, low cost, portable test kit for the detection and identification of chemical and biological agents

• US 10,330,603 B1
• Filed: 08/07/2017
• Issued: 06/25/2019
• Est. Priority Date: 04/08/2016
• Status: Active Grant

• Current Assignee: S2 Detection {Nevada}, Inc.
• Original Assignee: Veriteque USA Incorporated
• Inventors: Callahan, Michael D.
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US15/670,227
• Time in Patent Office: 687 Days
• Field of Search: 422400, 422411, 422418, 422425, 422430, 436 92, 436164
• CPC Class Codes: G01N 1/02 Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 2001/027 field kits / quick test kits
• CPC Class Codes: G01N 2001/028 Sampling from a surface, swabbing, vaporising
• CPC Class Codes: G01N 2021/7759 Dipstick; Test strip
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

A highly portable, paper and swab-based detection kit is provided for identifying chemical and biological agents. A method of mass manufacture providing low cost kits with long term commercial shelf life and a method of use is also provided.

Claims

1. A portable detection kit for identifying the presence of a target chemical or biological agent, comprising:

a first homogenous suspension of a first dry chemical powder colorimetric reagent (CR) mixed with an inert liquid diluent, deposited and dried onto at least a first portion of a solid support article;

a swab device pre-wetted with a solvent to facilitate the collection and transfer of a suspected chemical or biological agent residue to the coated solid support article and upon contacting the residue with the coated solid support article and mixing all components together on the coated solid support article, a known visual colorimetric indication is produced identifying a class of an unknown target chemical or biological agent present in the suspect residue; and

a light, air, and moisture proof package into which the coated solid support article and the swab device are individual hermetically sealed prior to use.

2. The portable detection kit as in claim 1, wherein the first CR comprises a dry micronized powder which undergoes a characteristic color change when combined with a target chemical agent.

3. The portable detection kit as in claim 2, wherein the first CR is selected from the group consisting of:

cresol red, methyl violet, crystal violet, ethyl violet, malachite green, methyl green, 2-(p-dimethylaminophenylazo)pyridine, paramethyl red, metanil yellow, 4-phenylazodiphenylamine, metacresol purple, orange IV, 4-o-tolylazo-o-toluidine, quinaldine red, 4,4′

-bis(4-amino-1-naphthylazo)-2,2′

-stilbenedisulfonic acid, p-naphtholbenzein, phenolphthalein, o-cresolphthalein, ethyl bis(2,4-dimethylphenyl)ethanoate, Thymolphthalein, alizarin yellow R, alizarin, p-(2,4-dihydroxyphenylazo) benzenesulfonic acid-sodium salt, 5,5′

-indigodisulfonic acid-disodium salt, 2,4,6-trinitrotoluene, 1,3,5-trinitrobenzene, and clayton yellow.

4. The portable detection kit as in claim 2, wherein the first CR is selected from the group consisting of:

merocyanine dyes, 4-[2-N-substituted-1,4-hydropyridin-4-ylidine)ethylidene]cyclohexa-2,5-di-en-1-one, red pyrazolone dyes, azomethine dyes, indoaniline dyes, diazamerocyanine dyes, indigoid dyes 2,6-diphenyl-4-(2,4,6-triphenylpyridinium-1-yl)phenolate, 1-ethyl-4-(methoxycarbonyl)pyridinium iodide,5-(dimethylamino)-5′

-nitro-2,2′

-bisthiophene, (2,4,6-triphenyl-I-pyridinio)-2,6-diphenylphenolate, alizarin red s.

5. The portable detection kit as in claim 4, wherein the first CR comprises a dry micronized powder which undergoes characteristic color change when combined with a target biological agent.

6. The portable detection kit as in claim 5, wherein the first CR is selected from the group consisting of:

acid fuschin, alcian blue 8gx, alizarin red s, 9-amino-6-chloro-2-methoxyacridine, aniline blue, auramine o, azure b, N,N′

-[1,2-ethanediylbis(oxy-2,1-phenylene)]bis[N-(carboxymethyl)-, benzothiazolium, 2,2′

-[1,3 propanediylbis[(dimethyliminio)-3,1-propanediyl-1 (4H)-pyridinyl-4-ylidenemethylidyne]]bis[3-methyl-, iodide (1;

4), brilliant cresyl blue, calcein, congo red, crystal violet, di-8-anepps, dihydroethidium, dihydrorhodamine 123, eosin b, fast green fcf, bicinchonininic acid, sodium carbonate, sodium potassium tartrate, sodium hydroxide, copper sulphate, pyrogallol red, sodium molybdate, succinic acid, sodium benzoate sodium oxalate, methanol, coomassie blue G-250, ethanol, phosphoric acid, and phosphomolybdate, phosphotungstate.

7. The portable detection kit as in claim 2, wherein the first CR is selected from the group consisting of:

6-amino-1-naphthol-3-sulphonic acid, zinc, diphenylbenzidine, phenylanthranilic acid, aniline sulfate, diphenylamine, ethylenediamine, N-1 naphthyl dihydrochloride, potassium iodide, bismuth nitrate, and sodium hydrogen sulphate, hexachloroplatinic(IV) acid hydrate.

8. The portable detection kit as in claim 2, wherein the first CR is selected from the group consisting of:

sodium iodoplatinate, Alkyl magnesium bromide (Grignard reagent), B-napthol, selenious acid, indophenol blue, silver nitrate, Grignard'"'"'s sodium iodide, sodium nitroprusside, quinone dichlorimide, mercuric nitrate, sodium iodide, N-chloramide, zinc Sulfate-Molybdic Acid, argentic nitrate, mercuric chloride, cuprous iodide, diphenylthiocarbazone, di-p-biphenylthiocarbazone, di-o-phenoxyphenylthiocarbazone, dianisylpropylene, p,p′

-dinitrostilbene-o,o′

-disodium sulfonate, 5-(or 8-) nitroisoquinoline, sodium iodoplatinate, chloroplatinic acid, phosphomolybdic acid, Mayer'"'"'s reagent (KHgI2), metanil-yellow, N-chloramide, diphenylthiocarbazone, potassium iodide and starch, acidified phloxine, p-dimethylaminobenzaldehyde and syn-trimethoxybenzene, p-nitrobenzyl bromide, 4-(4-nitrobenzyl)pyridine-tetraethylenepentamine, palladium chloride, bromophthalein, anisaldehyde, 4-aminopyridine, 2-methylthioacridone, trichlorobenzoquinoneimide, zinc hexacyanoferrate, cupric acetate, malachite green, and p,p′

-tetramethyldiaminodiphenylmethane (tetrabase).

9. The portable detection kit as in claim 1, wherein the inert liquid diluent is selected from the group consisting of acrylic acid, polyvinyl alcohol, amino cross-linking agents, polyvinyl pyrrolidone, glycol-ethers, styrene, polyester, vinyl chloride, polyethylene, natural gums, poly-ethers, and polyamides.

10. The portable detection kit as in claim 1, wherein the first homogeneous suspension further comprises an inert bulking agent.

11. The portable detection kit as in claim 1, wherein the swab device comprises:

a handle; and

an absorbent matrix collection tip for surface sample collection and physical mixing of the suspect residue and the first homogenous suspension.

12. The portable detection kit as in claim 11, wherein the inert bulking agent is selected from the group consisting of silica, diatomaceous earth, mixtures of liquid diluent, and combinations thereof.

13. The portable detection kit as in claim 1, further comprising a second homogenous suspension of a second CR deposited and dried onto a second portion of the solid support item separated from the first portion.

14. The portable detection kit as in claim 1, wherein the coated solid support article is formed into a strip.

15. The portable detection kit as in claim 1, wherein the solid support article is selected from the group consisting of glass, metal, paper, textiles, organic membranes, inorganic membranes, natural fibers, and synthetic fibers.

16. The portable detection kit as in claim 11, wherein the swab tip comprises a dry tip.

17. The portable detection kit as in claim 11, wherein the swab tip comprises a tip pre-wetted with a solvent.

18. The portable detection kit as in claim 17, wherein the pre-wetted solvent comprises an aqueous solvent or an organic solvent.

19. The portable detection kit as in claim 18, wherein the organic solvent is selected from the group consisting of alcohols, acetone, chlorinated hydrocarbons, dimethyl sulfoxide, and organic acids.

20. The portable detection kit as in claim 18, wherein the aqueous solvent is selected from the group consisting of water, mineral acids and alkali.

21. The portable detection kit as in claim 1, wherein the package comprises a layer of a PET or cellulosic material, a layer of aluminum, and a layer of a Poly Ethylene material.

22. The portable detection kit as in claim 21, wherein:

the layer of a PET or cellulosic material is approximately 12 microns thick;

the layer of aluminum is approximately 7 microns thick; and

the layer of a Poly Ethylene material is approximately 50 microns thick.

23. A method of providing a portable detection kit for identifying the presence of a target chemical or biological agent, comprising:

mixing a homogenous suspension of a dry chemical powder colorimetric reagent (CR) with an inert liquid diluent;

depositing the homogeneous suspension onto a solid support article;

drying the homogeneous suspension to coat the solid support article;

pre-wetting a swab device with a solvent to facilitate the collection and transfer of a suspected chemical or biological agent residue to the coated solid support article and upon contacting the residue with the coated solid support article and mixing all components together on the solid support article, a known visual colorimetric indication is produced thereby identifying a class of an unknown target chemical or biological agent present in the suspect residue; and

hermetically sealing the coated solid support article and the swab device in a light, air, and moisture proof package prior to use.

24. The method as in claim 23, further comprising processing the CR in a ball, crusher, or shaker mill to produce a sub-micron mesh size powder.

25. The method as in claim 24, further comprising mixing the CR with an inert liquid diluent in mixing containers and high speed shaker beds or in rotary impeller mixers.

26. The method as in claim 23, wherein depositing the homogenous suspension onto the solid support article comprises an automated commercial printing process.

27. The method as in claim 23, wherein the commercial printing process is selected from the group consisting of letterpress, rotary gravure, screen printing, tampography, wax printing, contact dosing, ultrasonic sputter, and drop on demand printing.

28. The method as in claim 23, wherein drying the deposited homogenous suspension comprises a process selected from the group consisting of UV, IR, and hot air cure.

29. The method as in claim 23, further comprising forming the coated solid support article into a predetermined shape by a process selected from the group consisting of injection molding, pressure forming, guillotining, and die-cutting.

30. The method as in claim 23, wherein depositing the homogenous suspension comprises depositing a plurality of homogenous suspensions, each comprising a different CR, onto the solid support article, the plurality of homogenous suspensions separated by physical voids therebetween.

31. The method as in claim 23, further comprising hermetically sealing the coated solid support article and the swab device in the package by vertical or horizontal form fill seal packaging systems.

32. The method according to claim 23, further comprising testing for the presence of a target chemical or biological agent by:

removing the coated solid support and swab device from the sealed package;

rubbing an object with the swab device to transfer a molecule or ion of an unknown suspect residue from the object to the swab device;

contacting the swab device with the homogenous suspension on the solid support article to transfer the molecule or ion to the homogenous suspension; and

rubbing the swab device through the homogenous suspension on the solid support article to mix together the suspect residue, the homogenous suspension, and the swab wetting solvent;

whereby a chemical reaction is facilitated to produce a presumptive colorimetric indication of the presence of a target chemical or biological agent if a target chemical or biological agent is present.