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Tissue processing apparatus and method for processing adipose tissue

  • US 10,336,980 B2
  • Filed: 09/05/2014
  • Issued: 07/02/2019
  • Est. Priority Date: 09/05/2013
  • Status: Active Grant
First Claim
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1. A portable apparatus with a container for multi-step processing of human biological material containing stringy tissue including washing, enzymatic digestion and centrifuging to prepare a cell concentrate in the container in the form of a pellet phase formed during the centrifuging, the apparatus being orientable in an access orientation and the apparatus comprising:

  • the container having an internal containment volume including a tissue retention volume and a filtrate volume;

    a filter disposed within the internal containment volume with the tissue retention volume on one side of the filter and the filtrate volume on another side of the filter with the tissue retention volume and the filtrate volume being in fluid communication through the filter;

    an inlet port in fluid communication with the tissue retention volume and configured to access the tissue retention volume for introducing human biological material into the tissue retention volume;

    a suction port in fluid communication with the filtrate volume and configured to access the filtrate volume for suctioning material from the filtrate volume;

    wherein;

    the internal containment volume includes a pellet well disposed in a bottom portion of the filtrate volume below a bottom elevation of the filter and accessible only from above when the apparatus is in the access orientation;

    the pellet well has a volume in a range of from 0.3 cubic centimeter to 3 cubic centimeters;

    when the apparatus is in the access orientation, the pellet well has at least one portion with a vertical length of 0.5 centimeter, a maximum horizontal cross-dimension of no larger than 10 millimeters and a minimum horizontal cross-dimension of no smaller than 1.5 millimeters; and

    the internal containment volume comprises an available processing volume with a portion of the available processing volume being in the tissue retention volume and the volume of the pellet well is in a range of from 0.2 percent to 2 percent of the portion of the available processingvolume in the tissue retention volume;

    wherein the available processing volume is in a range of from 20 cubic centimeters to 400 cubic centimeters.

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