Method for preparing organic acid by fed-batch-feeding carbon source substrate and base

• US 10,337,035 B2
• Filed: 05/21/2013
• Issued: 07/02/2019
• Est. Priority Date: 05/23/2012
• Status: Active Grant

• Current Assignee: SK Energy Corporation (SK Innovation Company Limited), SK Innovation Company Limited
• Original Assignee: SK Innovation Company Limited
• Inventors: Park, Jae Yeon, Kang, Sin Young, Park, Woo Chan, Koo, Min Su, Cho, In Ho, Park, Joong Min, Lee, Seung Yeop, Kim, Dong Hyun
• Primary Examiner(s): Bowers, Erin M.
• Application Number:
  US14/402,980
• Publication Number:
  US 20150140615A1
• Time in Patent Office: 2,233 Days
• Field of Search: None
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: C12P 7/46 Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• CPC Class Codes: C12P 7/52 Propionic acid; Butyric acids
• CPC Class Codes: C12P 7/54 Acetic acid vinegar C12J
• CPC Class Codes: C12P 7/56 Lactic acid

Abstract

The present invention provides a fed-batch culture method comprising a step of fed-batch-feeding a carbon source base and a base in such a manner that the pH level can be maintained at a level suitable for the growth of microorganisms for fermentation of a carbon source. The present invention also provides a method for preparing organic acids using the fed-batch culture method. The present invention fed-batch-feeds a neutralizing agent such as ammonium bicarbonate, ammonium carbonate or alkali metal-containing weak base, and a carbon source substrate in preparing organic acids by microorganism fermentation. Thus, a pH level suitable for the survival of microorganisms for carbon source fermentation can be maintained, and the speed of injecting the carbon source base which is the source material can be appropriately adjusted. The present invention may improve productivity, yield rate and concentration of organic acids and may automatically inject a base and a carbon source substrate according to a variation in the pH level, thus improving reliability and convenience of a fermentation process operation.

Claims

1. A method for producing an organic acid by fed-batch culture, comprising:

(i) preparing a culture medium containing a carbon source substrate, an organic acid-producing strain of bacteria, and a growth ingredient for said strain of bacteria;

(ii) fermenting said culture medium to produce said organic acid;

(iii) measuring a pH change in said culture medium;

(iv) correlating said pH change to consumption of said carbon source substrate;

(v) fed-batch feeding additional substrate-base mixture of carbon source substrate and an alkali metal containing weak base to said culture medium in an amount to maintain concentration of said carbon source substrate, and said alkali metal containing weak base in an amount to matain optimal pH for growth of said strain of bacteria, and

(vi) removing any organic acid produced in said culture medium;

wherein ratio of carbon source substrate to alkali metal containing weak base is 200˜

300 g/L to 80˜

120 g/L,

wherein CO₂ and H₂ gases are generated during said fermenting, and when CO₂ and H₂ gases reach a generation rate of 1.5-2.5 L/L of fermenter/hr, terminating the feeding the substrate-base mixture so that said fermenting proceeds until said carbon source substrate is completely consumed.

2. The method of claim 1, wherein, after completion of the feeding of the substrate-base mixture, ammonia water, alkali metal hydroxide solution, alkaline earth metal hydroxide solution, or a combination thereof is supplied to said fermenter to allow the culture medium to have a pH value optimal for growth of the organic acid-producing strain until the carbon source substrate is completely fermented.

3. The method of claim 1, wherein the growth ingredient is at least one selected from the group consisting of a nitrogen source, a vitamin, a mineral, and a carbon source degrading enzyme.

6. The method of claim 1, wherein said carbon source substrate is selected from the group consisting of glucose, fructose, sucrose, galactose, xylose, arabinose, sugar cane, molasses, and starch hydrolysate.

7. The method of claim 1, wherein the organic acid is at least one selected from the group consisting of butyric acid, lactic acid, acetic acid, formic acid, citric acid, adipic acid, succinic, acid, fumaric acid, malic acid, 3-hydroxypropionic acid, glutamic acid, glutaric acid, glucaric acid, itaconic acid, acrylic acid, and muconic acid.

8. The method of claim 1, wherein the organic acid-producing strain is selected from the group consisting of Clostridium spp., Pseudomonas spp., Rhizopus spp., Aspergillus spp., Corynebacterium spp., Actinobacillus spp., yeast, Candida yeast, Pichia yeast, E. coli, and lactic acid producing bacteria.

9. The method of claim 1, comprising fermenting said culture medium in a fermenter equipped with a pH control sensor, a gas meter or both.

4. A method for producing butyric acid by fed-batch culture, comprising;

(i) preparing a culture medium containing a carbon source substrate, a butyric acid-producing strain of bacteria, and a growth ingredient for the strain of bacteria;

(ii) fermenting said culture medium to produce said butyric acid;

(iii) measuring a pH change in said culture medium;

(iv) correlating said pH change to consumption of said carbon source substrate;

(v) fed-batch feeding additional substrate-base mixture of carbon source substrate and at least one base selected from the group consisting of ammonium bicarbonate, sodium bicarbonate, potassium bicarbonate, ammonium carbonate, sodium carbonate, potassium carbonate and mixture thereof, in an amount to matain a pH of said culture medium to have a pH of 4.5 to 7; and

(vi) removing any butyric acid produced in said culture medium;

wherein ratio of carbon source substrate to said at least one base is 200˜

300 g/L to 80˜

120 g/L,

wherein CO₂ and H₂ gases are generated during said fermenting, and when CO₂ and H₂ gases reach a generation rate of 1.5-2.5 L/L of fermenter/hr, terminating feeding said substrate-base mixture so that said fermenting proceeds until the carbon source substrate is completely consumed.

5. The method of claim 4, wherein said butyric acid-producing strain of bacteria is a Clostridium sp. microorganism.