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Labeling hydroxymethylated residues

DC
  • US 10,337,053 B2
  • Filed: 06/19/2018
  • Issued: 07/02/2019
  • Est. Priority Date: 09/26/2008
  • Status: Active Grant
First Claim
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1. A method comprising:

  • (a) labeling covalently, a hydroxyl group on a hydroxymethylated residue in a mammalian nucleic acid to generate a labeled 5-hydroxymethylcytosine residue, wherein said labeling comprises glycosylating the hydroxyl group by employing an alpha-glucosyltransferase, a beta-glucosyltransferase, or a beta-glucosyl-alpha-glucosyl-transferase; and

    (b) sequencing said mammalian nucleic acid comprising said labeled hydroxymethylated residue.

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