Dynamic flux nucleic acid sequence amplification
First Claim
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1. A real-time dynamic flux method of nucleic acid sequence amplification, comprising:
- a. combining a pair of forward and reverse oligonucleotide primers with a target nucleic acid sequence to be amplified; and
b. amplifying the target nucleic acid sequence by thermocycling the pair of forward and reverse oligonucleotide primers and the target nucleic acid sequence within a 15°
C. temperature range defined by the melting temperature of the oligonucleotide primers and the melting temperature of the target nucleic acid sequence, andwherein thermocycling comprises;
i. denaturing the target nucleic acid sequence; and
ii. annealing of the forward and reverse oligonucleotide primers; and
iii. extension of the target nucleic acid sequence by the forward and reverse oligonucleotide primers,c. simultaneously detecting the amplified target nucleic acid sequence during said amplifying step.
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Abstract
Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.
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Citations
8 Claims
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1. A real-time dynamic flux method of nucleic acid sequence amplification, comprising:
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a. combining a pair of forward and reverse oligonucleotide primers with a target nucleic acid sequence to be amplified; and b. amplifying the target nucleic acid sequence by thermocycling the pair of forward and reverse oligonucleotide primers and the target nucleic acid sequence within a 15°
C. temperature range defined by the melting temperature of the oligonucleotide primers and the melting temperature of the target nucleic acid sequence, andwherein thermocycling comprises; i. denaturing the target nucleic acid sequence; and ii. annealing of the forward and reverse oligonucleotide primers; and iii. extension of the target nucleic acid sequence by the forward and reverse oligonucleotide primers, c. simultaneously detecting the amplified target nucleic acid sequence during said amplifying step. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification