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Methods and systems for nucleic acid sequencing validation, calibration and normalization

  • US 10,337,058 B2
  • Filed: 09/17/2015
  • Issued: 07/02/2019
  • Est. Priority Date: 02/19/2010
  • Status: Active Grant
First Claim
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1. A method of preparing a quality control for performing nucleic acid sample sequencing, comprising:

  • generating a set of synthetic nucleic acid sequences, wherein each synthetic nucleic acid sequence in the set of synthetic nucleic acid sequences differs and wherein each of the synthetic nucleic acid sequences in the set of synthetic nucleic acid sequences produces a predefined pattern of detectable signals during sequencing;

    attaching one or more synthetic nucleic acid sequences from the set of synthetic nucleic acid sequences to solid supports in plural groups of solid supports, wherein the plural groups of solid supports are divided into a plurality of subsets and wherein the one or more synthetic nucleic acid sequences are attached to the solid supports in the plural groups such that, when nucleic acid sample sequencing is performed, all possible color transitions are observed for consecutive ligation cycles for each subset of the plural groups of solid supports; and

    combining each group of solid supports with the synthetic nucleic acid sequences attached to create a control set of solid supports for performing nucleic acid sample sequencing.

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