Methods and systems for nucleic acid sequencing validation, calibration and normalization
First Claim
1. A method of preparing a quality control for performing nucleic acid sample sequencing, comprising:
- generating a set of synthetic nucleic acid sequences, wherein each synthetic nucleic acid sequence in the set of synthetic nucleic acid sequences differs and wherein each of the synthetic nucleic acid sequences in the set of synthetic nucleic acid sequences produces a predefined pattern of detectable signals during sequencing;
attaching one or more synthetic nucleic acid sequences from the set of synthetic nucleic acid sequences to solid supports in plural groups of solid supports, wherein the plural groups of solid supports are divided into a plurality of subsets and wherein the one or more synthetic nucleic acid sequences are attached to the solid supports in the plural groups such that, when nucleic acid sample sequencing is performed, all possible color transitions are observed for consecutive ligation cycles for each subset of the plural groups of solid supports; and
combining each group of solid supports with the synthetic nucleic acid sequences attached to create a control set of solid supports for performing nucleic acid sample sequencing.
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Abstract
A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.
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Citations
9 Claims
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1. A method of preparing a quality control for performing nucleic acid sample sequencing, comprising:
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generating a set of synthetic nucleic acid sequences, wherein each synthetic nucleic acid sequence in the set of synthetic nucleic acid sequences differs and wherein each of the synthetic nucleic acid sequences in the set of synthetic nucleic acid sequences produces a predefined pattern of detectable signals during sequencing; attaching one or more synthetic nucleic acid sequences from the set of synthetic nucleic acid sequences to solid supports in plural groups of solid supports, wherein the plural groups of solid supports are divided into a plurality of subsets and wherein the one or more synthetic nucleic acid sequences are attached to the solid supports in the plural groups such that, when nucleic acid sample sequencing is performed, all possible color transitions are observed for consecutive ligation cycles for each subset of the plural groups of solid supports; and combining each group of solid supports with the synthetic nucleic acid sequences attached to create a control set of solid supports for performing nucleic acid sample sequencing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification