Spectroscopic measurements with parallel array detector

• US 10,337,984 B2
• Filed: 01/11/2016
• Issued: 07/02/2019
• Est. Priority Date: 07/18/2013
• Status: Active Grant

• Current Assignee: COR Health Incorporated
• Original Assignee: Nueon Inc.
• Inventors: Messerschmidt, Robert G.
• Primary Examiner(s): Stock, Jr., Gordon J
• Application Number:
  US14/992,945
• Publication Number:
  US 20160123869A1
• Time in Patent Office: 1,268 Days
• CPC Class Codes: A61B 2562/046 in a matrix array
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0261 using optical means, e.g. infrared light
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/208 adapted to determine urine quantity, e.g. flow, volume
• CPC Class Codes: G01N 21/27 using photo-electric detection G01N21/31 takes precedence; circuits for computing concentration logarithmic circuits G06G7/24; photometric circuits in general G01J
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

A measurement apparatus comprises optical components arranged to provide parallel measurements of a biological sample. The parallel sample measurements provide improved accuracy with lower detection limit thresholds. The parallel measurements may comprise one or more of Raman spectroscopy measurements or infrared spectroscopy measurements. The parallel measurements can be combined with a light source. In many embodiments, the light source comprises one or more wavelengths corresponding to resonance frequencies of one or more molecules of the sample, such as wavelengths of ultraviolet light. The wavelengths of light corresponding to resonance frequencies can provide an increased signal to noise ratio. The parallel array optical configuration can be combined with wavelengths of light corresponding to resonance frequencies in order to provide increased measurement accuracy and detection of metabolites.

Claims

1. An apparatus to measure a sample, the apparatus comprising:

an area array detector to measure a plurality of spectra of the sample, the area array detector comprising a plurality of detector elements;

a light source to illuminate the sample with a light beam, the light source comprising a tunable laser to selectively transmit each of a plurality of wavelengths of light of a laser beam along an optical path extending to the area array detector;

a support to receive the sample;

imaging optics configured to image light from the sample on the support onto the area array detector; and

a processor comprising a tangible medium coupled to the area array detector and the light source and comprising instructions to measure each of the plurality of detector elements for each of the plurality of wavelengths in order to determine an amount of light energy absorbed with the sample for said each of the plurality of detector elements and said each of the plurality of wavelengths;

wherein integration times of said each of the plurality of detector elements overlap for said each of the plurality of wavelengths such that a plurality of infrared absorbance spectra are measured in parallel.

2. An apparatus as in claim 1, wherein the plurality of spectra comprise one or more of a plurality of Raman spectra or a plurality of infrared absorbance spectra.

3. An apparatus as in claim 1, wherein the area array detector comprises a plurality of detectors arranged along a plurality of rows and a plurality of columns.

4. An apparatus as in claim 1, wherein the light beam comprises an energy uniformity of about +/−

25% across an area of the sample illuminated with the light beam.

5. An apparatus as in claim 1, wherein the light source comprises ultraviolet light having wavelengths corresponding to resonance of molecules of the sample.

6. An apparatus as in claim 1, wherein the light source comprises one or more of ultraviolet light, visible light, near infrared light, or infrared light.

7. An apparatus as in claim 1, wherein the sample comprises a blood sample and the blood sample comprises one or more of an individual blood cell, a fluid of the blood sample, a hematocrit of the blood sample, or fluids of the hematocrit and the processor comprises instructions to measure the blood sample.

8. An apparatus as in claim 1, wherein the support comprises an optically transmissive structure to analyze a blood sample with an internal reflection mode of the optically transmissive structure.

9. An apparatus as in claim 8, wherein the blood sample comprises blood cells located on a surface of the optically transmissive structure.

10. An apparatus as in claim 8, wherein the optically transmissive structure comprises a diamond blade.

11. An apparatus as in claim 8, wherein the sample comprises a blood sample and wherein a lancing device comprises an optical window for direct analysis of the blood sample with internal reflection.

12. A method of measuring a sample, the method comprising:

illuminating a sample on a support with a measurement beam comprising a plurality of wavelengths of light of a laser beam from a tunable laser;

imaging light from the sample on the support onto an array detector comprising a plurality of detector elements; and

measuring a plurality of spectra with the array detector by measuring each of the plurality of detector elements for each of the plurality of wavelengths in order to determine an amount of light energy absorbed with the sample for said each of the plurality of detector elements and said each of the plurality of wavelengths;

wherein integration times of said each of the plurality of detector elements overlap for said each of the plurality of wavelengths such that a plurality of infrared absorbance spectra are measured in parallel.

13. A method as in claim 12, wherein the sample comprises a blood sample and the blood sample is analyzed with an internal reflection mode.

14. A method as in claim 12, wherein the sample comprises a blood sample and wherein a lancing device is used as optical window for direct analysis of the blood sample, either in transmission or internal reflection mode.

15. A method as in claim 12, wherein the sample comprises a blood sample.

16. A method as in claim 15, the blood sample comprises one or more of an individual blood cell, a fluid of the blood sample, a hematocrit of the blood sample, or fluids of the hematocrit.

17. A method as in claim 15, wherein the blood sample is analyzed with an internal reflection mode of an optically transmissive structure.

18. A method as in claim 17, wherein the blood sample comprises blood cells located on a surface of the optically transmissive structure.

19. A method as in claim 17, wherein the optically transmissive structure comprises a diamond blade.