Cartridge assembly

• US 10,343,160 B2
• Filed: 10/11/2017
• Issued: 07/09/2019
• Est. Priority Date: 10/14/2016
• Status: Active Grant

• Current Assignee: Illumina Singapore Pte. Ltd. (Illumina Incorporated)
• Original Assignee: Illumina Incorporated
• Inventors: Lemoine, Richard L., Osmus, James, Lin, Sz-Chin Steven, Ang, Beng Keong
• Primary Examiner(s): Levkovich, Natalia
• Application Number:
  US15/729,729
• Publication Number:
  US 20180117587A1
• Time in Patent Office: 636 Days
• Field of Search: 422554, 422417
• CPC Class Codes: B01L 2200/02 Adapting objects or devices to another
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: G01N 21/00 Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light G01N3/00 - G01N19/00 take precedence
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/75 Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73

Abstract

A cartridge assembly that comprises a housing, including a flow cell chamber to receive a flow cell, and a well plate having liquid wells to receive desired amounts of liquids. The well plate includes a valve station, a pump station and a fluidics analysis station, and channels associated therewith. A pump assembly to manage fluid flow through the channels between the pump station and the fluidics analysis station. A rotary valve assembly that includes a rotor shaft and rotor valve positioned to rotate about a rotational axis and to selectively couple the wells to the pump station. The rotor shaft includes a dual spline configuration at the distal end thereof. The dual spline configuration has first and second sets of splines. The first set of splines forms a drive interface and the second set of splines forms a position encoding interface.

Claims

1. A cartridge assembly, comprising:

a housing including a flow cell chamber to receive a flow cell;

a well plate having liquid wells to receive amounts of liquids, the well plate including a valve station, a pump station and a fluidics analysis station, the well plate including channels fluidly connected with the wells, the valve station, pump station and fluidics analysis station;

a pump assembly provided on the well plate at the pump station, the pump assembly to manage fluid flow through the channels between the pump station and the fluidics analysis station; and

a rotary valve assembly positioned on the well plate at the valve station, the rotary valve assembly including a rotor shaft and a rotor valve positioned to rotate about a rotational axis and to selectively couple the wells to the pump station,

the rotor shaft having a distal end exposed through the housing,

the rotor shaft including a dual spline configuration at the distal end thereof, the dual spline configuration having first and second sets of splines, the first set of splines forming a drive interface, the second set of splines forming a position encoding interface.

2. The cartridge assembly of claim 1, wherein the distal end of the rotor shaft extends into a shaft well provided in the housing, thereby exposing the dual spline configuration to a valve drive assembly of a fluidics analysis instrument.

3. The cartridge assembly of claim 1, wherein the first set of splines are exterior splines extending about an exterior of the distal end of the rotor shaft, wherein lateral sides of adjacent splines are separated by a first predetermined spline to spline spacing, the first predetermined spline to spline spacing corresponds to a spline pattern on a drive shaft of a valve drive assembly.

4. The cartridge assembly of claim 1, wherein the second set of splines are interior splines formed about an interior of a cavity provided at the distal end of the rotor shaft, the interior splines having lateral sides that are angled such that adjacent lateral sides form a predetermined non-parallel angle with respect to one another, wherein the adjacent lateral sides merge at a bottom of the interior splines to form pockets to receive mating splines on a drive shaft of a valve drive assembly, the position encoding interface utilized by the valve drive assembly to track a position of the rotor shaft.

5. The cartridge assembly of claim 1, wherein the rotor valve is mounted to a proximal end of the rotor shaft through a coupling flange, the coupling flange to allow a predetermined amount of tilting movement between the rotor valve and rotor shaft.

6. The cartridge assembly of claim 1, wherein the rotor valve is mounted to a proximal end of the rotor shaft through a coupling flange, the rotor valve including a rotor base having one or more ribs positioned about a proximal end of the rotor shaft, the coupling flange held between the ribs and the proximal end of the rotor shaft.

7. The cartridge assembly of claim 1, wherein rotor valve includes a well plate engaging face having a central port and a radial port, the rotor valve including a channel oriented to extend in a radial direction outward from the central port to the radial port.

8. The cartridge assembly of claim 1, wherein the rotor valve includes a well plate engaging face having a central port and a radial port, wherein the central port is aligned to correspond with a rotational axis of the rotor shaft and to align with a central feed port in the well plate, the rotor valve to rotate about the rotational axis to align the radial port with a corresponding well port.

9. The cartridge assembly of claim 1, wherein the rotary valve includes a well plate engaging face formed with an interface ring thereon, the interface ring extending about a perimeter of the well plate engaging face.

10. The cartridge assembly of claim 1, further comprising:

a valve cap including an interior cavity to rotatably receive the rotary valve, the valve cap including one or more latch arms to secure the valve cap to the wells and downward against the well plate; and

a biasing element provided within the interior cavity and to apply a biasing force against the rotary valve to maintain a sealed interface between ports in the rotary valve and ports in the well plate.

11. The cartridge assembly of claim 1, wherein the pump assembly includes a plunger having a drive end and a biasing surface located at opposite ends of the plunger, the drive end and biasing surface exposed at upper and lower surfaces of the housing such that corresponding unidirectional drive and biasing forces are applied thereto in connection with moving the plunger in a reciprocating motion.

12. The cartridge assembly of claim 11, wherein the plunger has a drive arm and a plunger arm joined with one another through a bridge segment in a U-shape and are formed together in a monolithic structure, the drive and plunger arms to be received within support posts located on the well plate.

13. The cartridge assembly of claim 1, wherein the first set of splines have beveled edges to facilitate alignment of a drive shaft of a valve drive assembly with the distal end of the rotor shaft.

14. The cartridge assembly of claim 4, wherein the lateral sides of each spline in the second set of splines extend in a V-shape to form a 30 degree angle.

15. The cartridge assembly of claim 4, wherein the drive shaft operates at an angle to the rotational axis of the rotor shaft.

16. The cartridge assembly of claim 1, wherein the well plate includes well transition ports arranged in a predetermined pattern corresponding to the rotary valve assembly.