Methods and compositions for providing resistance to glufosinate

• US 10,344,295 B2
• Filed: 12/18/2015
• Issued: 07/09/2019
• Est. Priority Date: 12/19/2014
• Status: Active Grant

• Current Assignee: Agbiome,Inc. (AgBiome LLC)
• Original Assignee: Agbiome,Inc. (AgBiome LLC)
• Inventors: Beilinson, Vadim, Henriksen, James R., Jones, Janice C., Kelly, Rebekah Deter, Shekita, Amy
• Primary Examiner(s): O Hara, Eileen B
• Application Number:
  US15/531,010
• Publication Number:
  US 20170362604A1
• Time in Patent Office: 1,299 Days
• Field of Search: None
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: C12N 15/8209 Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
• CPC Class Codes: C12N 15/8277 Phosphinotricin
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12Y 203/01183 Phosphinothricin acetyltransferase (2.3.1.183)

Abstract

Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Claims

1. An isolated polynucleotide or a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having

(a) at least 97% identity to the amino acid sequence set forth in SEQ ID NO:

7, wherein said polypeptide confers glufosinate resistance to a host cell;

(b) at least 99% identity to the amino acid sequence set forth in SEQ ID NO;

9, wherein said polypeptide confers glufosinate resistance to a host cell;

or

(c) at least 90% identity to the amino acid sequence set forth in SEQ ID NO;

11, wherein said polypeptide confers glufosinate resistance to a host cell;

wherein said recombinant DNA comprises a heterologous promoter operably linked to the nucleotide sequence.

2. The isolated polynucleotide or recombinant DNA of claim 1, wherein said nucleotide sequence encodes the amino acid sequence set forth in SEQ ID NO:

7, 9 or 11.

3. The isolated polynucleotide or recombinant DNA construct of claim 1, comprising a nucleotide sequence encoding a polypeptide having at least 95% identity to the amino acid sequence set forth in SEQ ID NO:

11, wherein said polypeptide confers glufosinate resistance to a host cell.

4. The isolated polynucleotide or recombinant DNA construct of claim 1, comprising a nucleotide sequence encoding a polypeptide having at least 98% identity to the amino acid sequence set forth in SEQ ID NO:

11, wherein said polypeptide confers glufosinate resistance to a host cell.

5. The isolated polynucleotide or recombinant DNA construct of claim 1, wherein said recombinant polynucleotide construct further comprises a plasmid.

6. A plant cell comprising a heterologous polynucleotide comprising

a recombinant polynucleotide construct comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:

3, 7 or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;

9, wherein said polypeptide confers glufosinate resistance to the cell.

7. The plant cell of claim 6, wherein said nucleotide sequence encodes the amino acid sequence set forth in SEQ ID NO:

3, 7, 9 or 11.

8. The plant cell of claim 6, wherein said nucleotide sequence encodes an amino acid sequence having at least 95% sequence identity to SEQ ID NO:

3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;

9.

9. The plant cell of claim 6, wherein the plant cell exhibits enhanced resistance to glufosinate as compared to a wild type plant cell of the same species, strain or cultivar.

10. The plant cell of claim 6, wherein said plant cell is from a monocot.

11. The plant cell of claim 6, wherein said plant cell is from a dicot.

12. A plant comprising a plant cell of claim 6.

13. A plant explant comprising a plant cell of claim 6.

14. A transgenic seed comprising the heterologous polynucleotide produced by the plant of claim 12.

21. A method for controlling weeds in a field containing a crop comprising:

(a) applying to an area of cultivation, a crop or a weed in an area of cultivation a sufficient amount of glufosinate to control weeds without significantly affecting the crop; and

,

(b) planting the field with the plant of claim 12 or a transgenic seed produced from said plant.

15. A method of producing a plant cell having a heterologous polynucleotide encoding a polypeptide which confers glufosinate resistance to the plant cell comprising transforming said plant cell with

a) an isolated polynucleotide or a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:

3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;

9, wherein said polypeptide confers glufosinate resistance to the cell;

or,

b) a recombinant polynucleotide construct comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO;

3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;

9, wherein said polypeptide confers glufosinate resistance to the cell.

16. The method of claim 15, further comprising selecting a cell which is resistant to glufosinate by growing the transgenic plant or plant cell in the presence of a concentration of glufosinate under conditions where the polypeptide is expressed at an effective level, whereby the transgenic cell grows at a rate that is discernibly greater than the cell would grow if it did not contain the nucleic acid construct.

17. The method of claim 15, wherein said polynucleotide construct further comprises a polynucleotide sequence of interest.

18. The method of claim 16, where said cell is a plant cell.

19. The method of claim 18, wherein said plant cell is from a monocot.

20. The method of claim 18, wherein said plant cell is from a dicot.

22. A host cell comprising a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having

(a) at least 97% identity to the amino acid sequence set forth in SEQ ID NO:

7, wherein said polypeptide confers glufosinate resistance to a host cell;

(b) at least 99% identity to the amino acid sequence set forth in SEQ ID NO;

9, wherein said polypeptide confers glufosinate resistance to a host cell;

or

(c) at least 90% identity to the amino acid sequence set forth in SEQ ID NO;

11, wherein said polypeptide confers glufosinate resistance to a host cell;

wherein said recombinant DNA comprises a heterologous promoter operably linked to the nucleotide sequence.