Methods and compositions for providing resistance to glufosinate
First Claim
1. An isolated polynucleotide or a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having(a) at least 97% identity to the amino acid sequence set forth in SEQ ID NO:
- 7, wherein said polypeptide confers glufosinate resistance to a host cell;
(b) at least 99% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to a host cell;
or(c) at least 90% identity to the amino acid sequence set forth in SEQ ID NO;
11, wherein said polypeptide confers glufosinate resistance to a host cell;
wherein said recombinant DNA comprises a heterologous promoter operably linked to the nucleotide sequence.
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Accused Products
Abstract
Compositions and methods comprising polynucleotides and polypeptides that confer glufosinate resistance to a host cell are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the sequence that confer glufosinate resistance. Various methods of employing these sequences are provided. Such methods include, for example, methods for producing a host cell, plant, plant cell, explant or seed having glufosinate resistance, and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.
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Citations
22 Claims
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1. An isolated polynucleotide or a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having
(a) at least 97% identity to the amino acid sequence set forth in SEQ ID NO: - 7, wherein said polypeptide confers glufosinate resistance to a host cell;
(b) at least 99% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to a host cell;
or(c) at least 90% identity to the amino acid sequence set forth in SEQ ID NO;
11, wherein said polypeptide confers glufosinate resistance to a host cell;wherein said recombinant DNA comprises a heterologous promoter operably linked to the nucleotide sequence. - View Dependent Claims (2, 3, 4, 5)
- 7, wherein said polypeptide confers glufosinate resistance to a host cell;
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6. A plant cell comprising a heterologous polynucleotide comprising
a recombinant polynucleotide construct comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO: - 3, 7 or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to the cell. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 21)
- 3, 7 or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;
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15. A method of producing a plant cell having a heterologous polynucleotide encoding a polypeptide which confers glufosinate resistance to the plant cell comprising transforming said plant cell with
a) an isolated polynucleotide or a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO: - 3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to the cell;
or,b) a recombinant polynucleotide construct comprising a nucleotide sequence encoding a polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO;
3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to the cell. - View Dependent Claims (16, 17, 18, 19, 20)
- 3, 7, or 11 or at least 96% identity to the amino acid sequence set forth in SEQ ID NO;
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22. A host cell comprising a recombinant DNA comprising a nucleotide sequence encoding a polypeptide having
(a) at least 97% identity to the amino acid sequence set forth in SEQ ID NO: - 7, wherein said polypeptide confers glufosinate resistance to a host cell;
(b) at least 99% identity to the amino acid sequence set forth in SEQ ID NO;
9, wherein said polypeptide confers glufosinate resistance to a host cell;
or(c) at least 90% identity to the amino acid sequence set forth in SEQ ID NO;
11, wherein said polypeptide confers glufosinate resistance to a host cell;wherein said recombinant DNA comprises a heterologous promoter operably linked to the nucleotide sequence.
- 7, wherein said polypeptide confers glufosinate resistance to a host cell;
Specification