Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
First Claim
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1. A recombinant Chlorella or Prototheca cell engineered to ablate or downregulate expression of an endogenous fatty acyl-ACP thioesterase gene.
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Abstract
Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.
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7 Claims
- 1. A recombinant Chlorella or Prototheca cell engineered to ablate or downregulate expression of an endogenous fatty acyl-ACP thioesterase gene.
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