Cucurbita plant resistant to potyvirus

• US 10,351,874 B2
• Filed: 04/08/2012
• Issued: 07/16/2019
• Est. Priority Date: 04/20/2011
• Status: Active Grant

• Current Assignee: Syngenta Participations AG (China National Chemical Corporation)
• Original Assignee: Syngenta Participations AG (China National Chemical Corporation)
• Inventors: Nicolas, Matthieu, Nicolet, Jean-Louis, Oliver, Marc, Danan, Sarah
• Primary Examiner(s): Stankovic, Bratislav
• Application Number:
  US14/112,657
• Publication Number:
  US 20140115735A1
• Time in Patent Office: 2,655 Days
• Field of Search: None
• CPC Class Codes: A01H 5/08 Fruits
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention relates to a Cucurbita plant, in particular a squash plant, having wide spectrum resistance to potyvirus such as Zucchini Yellow Mosaic Virus (ZYMV), Watermelon Mosaic Virus (WMV), Papaya Ringspot Virus (PRSV) and Moroccan watermelon mosaic virus (MWMV). Methods of selecting a squash plant having wide spectrum potyvirus resistance by marker assisted breeding are also provided.

Claims

1. A cultivated Cucurbita pepo plant comprising a recessive genetic determinant which is capable of directing or controlling resistance to potyvirus, wherein said genetic determinant is a genetic determinant of Cucurbita pepo cv. 268NiW, representative seed of which is deposited at NCIMB under accession number NCIMB 41727, and said genetic determinant is:

a genetic determinant that is genetically linked to marker locus Ni+, which marker locus comprises a single nucleotide polymorphism (SNP) and co-segregates with the potyvirus resistance trait and said marker locus can be identified in the genome of said plant in a PCR by amplification of a DNA fragment containing the marker locus with the pair of oligonucleotide primers;

forward primer of SEQ ID NO;

13 and reverse primer of SEQ ID NO;

14 followed by detection of the marker locus with resistant allele specific probe SEQ ID NO;

15, wherein the marker comprises a nucleotide C corresponding to position 13 of SEQ ID NO;

15.

2. The plant according to claim 1, wherein the plant is a squash plant.

3. Seed of a squash plant according to claim 2 which is capable of growing a potyvirus resistant squash plant comprising the genetic determinant which is capable of directing or controlling resistance to potyvirus.

4. The plant according to claim 1, wherein the plant is resistant to Zucchini Yellow Mosaic Virus (ZYMV).

5. The plant according to claim 1, wherein said plant further comprises:

a genetic determinant that is genetically linked to marker locus W1, which marker locus comprises a SNP and co-segregates with the potyvirus resistance trait and said marker locus can be identified in the genome of said plant in a PCR by amplification of a DNA fragment containing the marker locus with the pair of oligonucleotide primers;

forward primer of SEQ ID NO;

3 and reverse primer of SEQ ID NO;

4 followed by detection of the marker locus with resistant allele specific probe SEQ ID NO;

9, wherein the marker comprises a nucleotide C corresponding to position 10 of SEQ ID NO;

9; and

a genetic determinant that is genetically linked to marker locus W2, which marker locus comprises two SNPs and co-segregates with the potyvirus resistance trait and said marker locus can be identified in the genome of said plant in a PCR by amplification of a DNA fragment containing the marker locus with the pair of oligonucleotide primers;

forward primer of SEQ ID NO;

5 and reverse primer of SEQ ID NO;

6 followed by detection of the marker locus with resistant allele specific probe SEQ ID NO;

11, wherein the marker comprises a nucleotide T corresponding to position 7 and a nucleotide C corresponding to position 9 of SEQ ID NO;

11.

6. The plant according to claim 5, wherein the plant is a squash plant.

7. Seed of a squash plant according to claim 6 which is capable of growing a potyvirus resistant squash plant comprising the genetic determinants which are capable of directing or controlling resistance to potyvirus.

8. The plant according to claim 5, wherein the plant is resistant to MWMV, PRSV, WMV and/or ZYMV.

9. The plant according to claim 5, wherein the plant is resistant to MWMV and ZYMV.

10. The plant according to claim 5, wherein the plant is resistant to MWMV, PRSV, WMV and ZYMV.

11. The plant according to claim 1, wherein the plant is resistant to ZYMV and WMV.

12. The plant according to claim 1, wherein the plant is resistant to ZYMV.

13. The plant according to claim 1, wherein said plant further comprises:

a genetic determinant that is genetically linked to marker locus ZN, which marker locus comprises a single nucleotide polymorphism (SNP) and co-segregates with the potyvirus resistance trait and said marker locus can be identified in the genome of said plant in a PCR by amplification of a DNA fragment containing the marker locus with the pair of oligonucleotide primers;

forward primer of SEQ ID NO;

1 and reverse primer of SEQ ID NO;

2 followed by detection of the marker locus with resistant allele specific probe SEQ ID NO;

7, wherein the marker comprises a nucleotide C corresponding to position 6 of SEQ ID NO;

7.

14. The plant according to claim 13, wherein the plant is a squash plant.

15. Seed of a squash plant according to claim 14 which is capable of growing a potyvirus resistant squash plant comprising the genetic determinants which are capable of directing or controlling resistance to potyvirus.

16. The plant according to claim 13, wherein the plant is resistant to ZYMV.

17. The plant according to claim 13, wherein the plant is resistant to MWMV and ZYMV.