Reagent cartridge for detection of cells

• US 10,351,893 B2
• Filed: 10/03/2016
• Issued: 07/16/2019
• Est. Priority Date: 10/05/2015
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: GeneWEAVE BioSciences, Inc. (Roche Holding AG)
• Inventors: Donnelly, Doran, Frei, Werner, Griswold, Ryan, Page, Lance, Roy, Shaunak
• Primary Examiner(s): Levkovich, Natalia
• Application Number:
  US15/283,797
• Publication Number:
  US 20170096697A1
• Time in Patent Office: 1,016 Days
• Field of Search: 422550, 422570, 422921
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: C12Y 113/12007 Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
• CPC Class Codes: G01N 2333/195 from bacteria
• CPC Class Codes: G01N 2333/90241 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Abstract

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.

Claims

1. An apparatus, comprising:

a sample tube;

a housing having a connection portion configured to mate with the sample tube, the housing further including a side wall defining an inner reagent volume of the housing, the housing further comprising a top opening into the inner reagent volume, the housing further including a bottom end wall that separates the inner reagent volume from the sample tube when the housing is coupled to the sample tube, the bottom end wall comprising a puncturer having a sharp point, the bottom end wall further comprising a through hole that defines a delivery path between the inner reagent volume and the sample tube, the bottom end wall further including a plurality of protrusions projecting inwardly from a side surface of the through hole in the bottom end wall into the delivery path;

a blister container within the inner reagent volume, the blister container containing a reagent and including a frangible portion; and

an actuator having a plunger coupled to an engagement portion, the engagement portion accessible through the top opening, the engagement portion configured to be manipulated to move the plunger within the inner reagent volume such that the puncturer pierces the frangible portion of the blister container to convey the reagent from the inner reagent volume into the sample tube via the delivery path.

2. The apparatus of claim 1, wherein:

the bottom end wall defines an exit opening at the end of the through hole through which the reagent is conveyed when the reagent exits the delivery path into the sample tube; and

the plurality of protrusions project inwardly from the side surface of the through hole in the bottom end wall into the exit opening.

3. The apparatus of claim 1, wherein:

the delivery path defines a longitudinal center line and has a path length along the longitudinal center line; and

a protrusion from the plurality of protrusions includes an edge parallel to the longitudinal center line, the edge having a protrusion length of at least ten percent of the path length.

4. The apparatus of claim 1, wherein:

the delivery path defines a longitudinal center line and has a path length along the longitudinal center line; and

a protrusion from the plurality of protrusions includes an edge parallel to the longitudinal center line, the edge having a protrusion length of at least half of the path length.

5. The apparatus of claim 4, wherein the protrusion length s substantially equal to the path length.

6. The apparatus of claim 1, wherein:

the delivery path defines a longitudinal center line and a flow area, the flow area bounded by the side surface of the through hole and being within a plane normal to the longitudinal center line, the flow area of the delivery path having a diameter; and

a protrusion from the plurality of protrusions extends inwardly from the side surface of the through hole in the bottom end wall into the flow area by a distance from the side surface, a ratio of the distance to the diameter being between about 0.1 and 0.2.

7. The apparatus of claim 1, wherein:

the delivery path defines a longitudinal center line, a path length, and a flow area, the path length being along the longitudinal center line, the flow area bounded by the side surface of the through hole and being within a plane normal to the longitudinal center line, the flow area of the delivery path having a diameter, a ratio of the path length to the diameter being between about 2.5 and about 3.5.

8. The apparatus of claim 1, wherein:

the delivery path defines a longitudinal center line; and

the plurality of protrusions are equally spaced circumferentially about the longitudinal center line.

9. The apparatus of claim 1, wherein

the reagent is formulated to react with a plurality of reporter molecules in a sample to enhance production of a signal.

10. The apparatus of claim 1, wherein:

the reagent is a first reagent formulated to react with a plurality of reporter molecules in a sample to enhance production of a signal; and

the sample tube containing a second reagent formulated to react with t sample to limit production of the signal.

11. An apparatus, comprising:

a sample tube;

a housing having a connection portion configured to mate with the sample tube, the housing further including a side wall defining an inner reagent volume of the housing, the housing further comprising a top opening into the inner reagent volume, the housing further including a bottom end wall that separates the inner reagent volume from the sample tube when the housing is coupled to the sample tube, the bottom end wall comprising a puncturer having a sharp point, the bottom end wall further comprising a through hole that defines a delivery path between the inner reagent volume and the sample tube, a lower-most surface of the bottom end wall defining an exit opening of the through hole through which an exit flow of a reagent is conveyed when exiting the delivery path, the bottom end wall further including a plurality of protrusions projecting inwardly from a side surface of the through hole in the bottom end wall into the exit opening;

a blister container within the inner reagent volume, the blister container containing the reagent and including a frangible portion; and

an actuator having a plunger coupled to an engagement portion, the engagement portion accessible through the top opening, the engagement portion configured to be manipulated such that the puncturer pierces the frangible portion of the blister container to produce the exit flow of the reagent through the delivery path and the exit opening.

12. The apparatus of claim 11, wherein:

the delivery path defines a longitudinal center line and a flow area, the flow area bounded by the side surface of the through hole and being within a plane normal to the longitudinal center line, the flow area of the delivery path having a diameter; and

a protrusion from the plurality of protrusions extends inwardly from the side surface of the through hole in the bottom end wall into the exit opening by a distance from the side surface, a ratio of the distance to the diameter being between about 0.1 and 0.2.

13. The apparatus of claim 11, wherein the plurality of protrusions project inwardly from the side surface of the through hole in the bottom end wall into the delivery path.

14. The apparatus of claim 13, wherein:

the delivery path defines a longitudinal center line and has a path length along the longitudinal center line; and

a protrusion from the plurality of protrusions includes an edge parallel to the longitudinal center line, the edge having an edge length of at least half of the path length.

15. The apparatus of claim 11, wherein the bottom end wall defines a flow area along a path length within the delivery path, the flow area bounded by the side surface of the through hole and being within a plane normal to a longitudinal center line of the delivery path, the plurality of protrusion positioned within the delivery path such that a shape of the flow area is discontinuous.

16. The apparatus of claim 11, wherein the plurality of protrusions are equally spaced circumferentially about a longitudinal center line of the delivery path.