Pathogenesis quantification systems and treatment methods for citrus greening blight
First Claim
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1. A method for detecting pathogenesis in a biological sample, comprising:
- a) quantifying an amount of a nucleic acid in the biological sample specific for a first organism that is a pathogen associated with a second organism, wherein in tandem the first and second organisms affect pathogenesis of a host organism, to determine a pathogen quantitative measurement,b) quantifying an amount of a nucleic acid in the biological sample specific for the second organism, to determine a second organism quantitative measurement;
c) quantifying an amount of a nucleic acid in the sample specific for the host organism response to the pathogen, to determine a multiplex quantitation for targets associated with the pathogen of a disease; and
d) calculating a ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures, wherein said ratio provides a pathogen index for detecting pathogenesis.
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Abstract
The invention relates to a novel pathogenesis model, method, or kit for detecting pathogenesis in a subject. In particular, the invention provides a pathogen index derived from a ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures. The pathogen index is used in diagnosis, prognosis, and/or treatment strategy of any disease, including citrus greening diseases (HLB). Research tools and methods for screening drugs for treating or preventing citrus greening diseases, as well as treatment or prevention methods for citrus greening diseases are also provided.
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10 Claims
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1. A method for detecting pathogenesis in a biological sample, comprising:
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a) quantifying an amount of a nucleic acid in the biological sample specific for a first organism that is a pathogen associated with a second organism, wherein in tandem the first and second organisms affect pathogenesis of a host organism, to determine a pathogen quantitative measurement, b) quantifying an amount of a nucleic acid in the biological sample specific for the second organism, to determine a second organism quantitative measurement; c) quantifying an amount of a nucleic acid in the sample specific for the host organism response to the pathogen, to determine a multiplex quantitation for targets associated with the pathogen of a disease; and d) calculating a ratio of the amount of dual pathogen targets relative to the amount of host quantitative measures, wherein said ratio provides a pathogen index for detecting pathogenesis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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