Method and apparatus for analyzing acetone in breath

• US 10,352,940 B2
• Filed: 12/01/2016
• Issued: 07/16/2019
• Est. Priority Date: 05/15/2012
• Status: Active Grant

• Current Assignee: Invoy Holdings, Inc.
• Original Assignee: Invoy Holdings, LLC
• Inventors: Ahmad, Lubna M., Satterfield, Brent C., Martineau, Rhett L.
• Primary Examiner(s): Mui, Christine T
• Application Number:
  US15/366,309
• Publication Number:
  US 20170176447A1
• Time in Patent Office: 957 Days
• Field of Search: 436130, 356433, 356432
• CPC Class Codes: A61B 2010/0087 Breath samples
• CPC Class Codes: A61B 2560/0233 Optical standards
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/082 Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath A61B5/083, A61B5/091 take precedence
• CPC Class Codes: A61B 5/097 Devices for facilitating collection of breath or for directing breath into or through measuring devices
• CPC Class Codes: G01N 21/783 for analysing gases
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: G01N 33/4975 other than oxygen, carbon dioxide or alcohol, e.g. organic vapours
• CPC Class Codes: G01N 33/64 involving ketones
• CPC Class Codes: Y10T 436/202499 Formaldehyde or acetone

Abstract

Methods and devices are provided for analyzing acetone in breath. One such method comprises disposing a reactant in a reaction zone within the breath analysis device, wherein the reactant comprises a primary amine disposed on a surface, and wherein the reaction zone has an optical characteristic that is at a reference level. It also comprises pre-storing a liquid nitroprusside solution within the breath analysis device separately from the reactant. The method further comprises using the breath analysis device to cause the breath to contact the reactant in the reaction zone so that the acetone in the breath reacts with the reactant to form a reaction product and, after the reaction product has been formed, using the breath analysis device to cause the nitroprusside solution to contact and react with the reaction product and to facilitate a change in the optical characteristic of the reaction zone relative to the reference level. The method also comprises using the breath analysis device to detect the change in the optical characteristic to sense the acetone in the breath. Apparatuses that use these methods are also described.

Claims

1. A method for sensing acetone in breath, the method comprising:

providing a cartridge comprising;

a reactant in a reaction zone, wherein the reactant comprises an amine disposed on a solid surface, and

a nitroprusside in a reaction zone, the nitroprusside disposed on a solid surface,

wherein the reaction zone has an optical characteristic that is at a reference level;

causing a breath sample to be passed through the cartridge such that the acetone in the breath sample reacts with the reactant to form a reaction product, wherein the reaction product is configured to react with the nitroprusside,

resulting in a change in the optical characteristic of the reaction zone relative to the reference level; and

measuring the change in the optical characteristic of the reaction zone, said change reflective of a concentration of acetone in the breath sample.

2. The method of claim 1, wherein causing the breath sample to be passed through the cartridge comprises pumping the breath sample through the cartridge.

3. The method of claim 1, wherein the solid surface comprises a silica gel.

4. The method of claim 1, wherein the solid surface comprises surfaces of a plurality of silica gel beads.

5. The method of claim 4, wherein the silica gel beads have a size distribution between 270 and 100 mesh.

6. The method of claim 1, further comprising maintaining the reactant in an alkaline environment.

7. The method of claim 1, wherein the amine is quenched with a non-volatile acid.

8. The method of claim 1, wherein the amine is quenched with sulfuric acid.

9. The method of claim 1, further comprising removing humidity from the breath sample before causing the breath sample to pass through the reaction zone of the cartridge.

10. The method of claim 9, wherein removing humidity from the breath sample comprises causing the breath sample to pass through a desiccant.

11. The method of claim 1, wherein the amine and the nitroprusside are disposed on the same solid surface together.

12. The method of claim 1, wherein the amine is a primary amine.

13. The method of claim 1, wherein the amine is aminopropyltriethoxysilane (APTES).

14. The method of claim 1, wherein the nitroprusside is sodium nitroprusside.

15. A breath analysis system, comprising:

a cartridge having a reaction zone containing a reactant, the reaction zone forming part of a flow path through the cartridge, the reactant comprising an amine disposed on a solid surface, the reaction zone further comprising a nitroprusside disposed on a solid surface, the reaction zone having an optical characteristic that is at a reference level, wherein the cartridge is configured such that, when a breath sample is passed along the flow path, acetone in the breath sample reacts with the reactant to form a reaction product, the reaction product configured to react with the nitroprusside resulting in a change in the optical characteristic of the reaction zone relative to the reference level; and

an optical sensor positioned to measure the change in the optical characteristic of the reaction zone, said change reflective of a concentration level of acetone in the breath sample.

16. The breath analysis system of claim 15, wherein the solid surface comprises a silica gel.

17. The breath analysis system of claim 15, wherein the solid surface comprises surfaces of a plurality of silica gel beads.

18. The breath analysis system of claim 17, wherein the silica gel beads have a size distribution between 270 and 100 mesh.

19. The breath analysis system of claim 15, wherein the amine is quenched with a non-volatile acid.

20. The breath analysis system of claim 15, wherein the amine is quenched with sulfuric acid.

21. The breath analysis system of claim 15, further comprising a desiccant for removing humidity from the breath sample.

22. The breath analysis system of claim 21, wherein the desiccant is selected from a group consisting of:

ascarite, sodium hydroxide and calcium chloride.

23. The breath analysis system of claim 15, wherein the amine and the nitroprusside are disposed on the same solid surface together.

24. The breath analysis system of claim 15, wherein the nitroprusside is disposed on the solid surface without formation of a chemical bond.

25. The breath analysis system of claim 15, wherein the amine is a primary amine.

26. The breath analysis system of claim 15, wherein the amine is aminopropyltriethoxysilane (APTES).

27. The breath analysis system of claim 15, wherein the nitroprusside is sodium nitroprusside.