Sensors for assaying coagulation in fluid samples
First Claim
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1. A method for performing one or more diagnostic clotting time tests on a blood sample, the method comprising:
- providing one or more sensors for performing the diagnostic clotting time tests, wherein each of the one or more sensors comprises at least one transducer coated with a polymer layer, wherein the polymer layer is a porous support layer that comprises immobilized therein a thrombin-cleavable peptide, wherein the thrombin-cleavable peptide comprises a detectable moiety linked by a thrombin-cleavable amide bond to a polypeptide sequence that is non-reactive with blood proteases other than thrombin;
contacting the blood sample with one or more reagents for performing the one or more diagnostic clotting time tests;
contacting the blood sample with the one or more sensors; and
determining the one or more diagnostic clotting times using at least the one or more sensors.
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Abstract
The present invention relates to analytical testing devices including micro-environment sensors and methods for assaying coagulation in a fluid sample applied to the micro-environment sensors, and in particular, performing one or more types of coagulation assays using one or more micro-environment sensors in a single point of care combined test cartridge. For example, the present invention may be directed to test sensor including at least one transducer coated with a polymer layer. The polymer layer comprises a thrombin-cleavable peptide with a detectable moiety.
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Citations
20 Claims
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1. A method for performing one or more diagnostic clotting time tests on a blood sample, the method comprising:
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providing one or more sensors for performing the diagnostic clotting time tests, wherein each of the one or more sensors comprises at least one transducer coated with a polymer layer, wherein the polymer layer is a porous support layer that comprises immobilized therein a thrombin-cleavable peptide, wherein the thrombin-cleavable peptide comprises a detectable moiety linked by a thrombin-cleavable amide bond to a polypeptide sequence that is non-reactive with blood proteases other than thrombin; contacting the blood sample with one or more reagents for performing the one or more diagnostic clotting time tests; contacting the blood sample with the one or more sensors; and determining the one or more diagnostic clotting times using at least the one or more sensors. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for performing two or more diagnostic clotting time tests on a blood sample, the method comprising:
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providing a test device comprising two or more sensors for performing the diagnostic clotting time tests, wherein each of the two or more sensors comprise at least one transducer coated with a polymer layer, wherein the polymer layer is a porous support layer that comprises immobilized therein a thrombin-cleavable peptide, wherein the thrombin-cleavable peptide comprises a detectable moiety linked by a thrombin-cleavable amide bond to a polypeptide sequence that is non-reactive with blood proteases other than thrombin; contacting in the test device the blood sample with reagents for performing each of the two or more diagnostic clotting time tests; contacting the blood sample in the test device with the two or more sensors; and determining the two or more diagnostic clotting times using at least the two or more sensors. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification