Methods of processing municipal solid waste (MSW) using microbial hydrolysis and fermentation

• US 10,358,370 B2
• Filed: 12/18/2013
• Issued: 07/23/2019
• Est. Priority Date: 06/12/2013
• Status: Active Grant

• Current Assignee: Renescience A/S
• Original Assignee: Renescience A/S
• Inventors: Ronsch, Georg Ornskov, Jensen, Jacob Wagner, Antonsen, Sebastian Buch
• Primary Examiner(s): Claytor, Renee
• Assistant Examiner(s): Papciak, Sharon M.
• Application Number:
  US14/897,963
• Publication Number:
  US 20160115063A1
• Time in Patent Office: 2,043 Days
• Field of Search: None
• CPC Class Codes: B09B 3/00 Destroying solid waste or transforming solid waste into something useful or harmless
• CPC Class Codes: B09B 5/00 Operations not covered by a single other subclass or by a single other group in this subclass
• CPC Class Codes: C02F 11/04 Anaerobic treatment; Production of methane by such processes
• CPC Class Codes: C02F 3/342 characterised by the enzymes used
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 5/023 Methane
• CPC Class Codes: C12P 7/08 produced as by-product or from waste or cellulosic material substrate
• CPC Class Codes: C12P 7/56 Lactic acid
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: C12R 2001/04 Actinomyces
• CPC Class Codes: C12R 2001/07 Bacillus
• CPC Class Codes: C12R 2001/08 Bacillus brevis
• CPC Class Codes: C12R 2001/10 Bacillus licheniformis
• CPC Class Codes: C12R 2001/125 Bacillus subtilis ; Hay bacillus; Grass bacillus
• CPC Class Codes: C12R 2001/225 Lactobacillus
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention provides a method of processing MSW comprising the steps of —providing a stream of unsorted MSW to a microbial fermentation reactor in which the MSW is fermented with agitation at a non-water content of between 10 and 50% by weight and at a temperature of between 35 and 75 degrees for a period of between 1 and 72 hours under conditions sufficient to maintain a live lactic acid bacteria concentration of at least 10.000.000.000 CFU/L, and —removing a stream of fermented unsorted MSW from the reactor and subjecting it to a separation step whereby non-degradable solids are removed to provide a slurry of bio-degradable components. Further, a slurry of bio-degradable components prepared according to said method is provided.

Claims

1. A method of processing Municipal Solid Waste (MSW) comprising the steps of

fermenting a stream of unsorted MSW in a microbial fermentation reactor in which the unsorted MSW is fermented with an inoculated microbial consortium and with agitation at a non-water content of between 10 and 50% by weight and at a temperature of between 35 and 75 degrees C. for a period of between 1 and 72 hours, under conditions sufficient to maintain a live lactic acid bacteria concentration of at least 1.0×

10¹⁰ CFU/L, wherein said microbial consortium comprises live lactic acid bacteria and wherein said microbial consortium provides a microbially-derived cellulase activity of at least 30 Filter Paper Units/Liter (FPU/L), and wherein said unsorted MSW comprises a mixture of biogenic and non-biogenic material in which 15% by weight or greater of the dry weight is non-biogenic material, and

removing a stream of fermented unsorted MSW from the reactor and subjecting it to a separation step whereby non-biodegradable solids are removed to provide a slurry of bio-degradable components.

2. The method of claim 1, wherein the unsorted MSW stream is inoculated with a microbial consortium of microorganisms naturally occurring in the waste, and propagated on local waste or components of local waste as a food source in fermentation conditions of temperatures within the range 37 to 55 degrees C., or 40 to 55 degrees C., or 45 to 50 degrees C., and at a pH within the range 4.2 and 6.0.

3. The method of claim 1, wherein water content has been added to the MSW in order to achieve a constant mass ratio of water between 0.5 and 2.5 kg water per kg MSW.

4. The method claim 1, wherein cellulase activity is added by inoculation with a selected microorganism that exhibits extra-cellular cellulase activity.

5. The method of claim 1, wherein the slurry of bio-degradable components is subject to post-fermentation following separation of non-degradable solids.

6. The method of claim 1, wherein inoculation of the unsorted MSW stream is provided by recycling wash waters or process solutions used to recover residual organic material from non-degradable solids and/or wherein inoculation of the unsorted MSW stream is provided before or concurrently with the addition of enzymatic activities.

7. The method of claim 6, wherein said recycled wash waters or process solutions have been enriched prior to inoculation of the microbial fermentation reactor, wherein the enriched recycled wash waters or process solutions are better able to promote bacterial growth compared to the recycled wash waters or process solutions prior to enrichment.

8. The method of claim 7, wherein said enrichment comprises incubation of the recycled wash waters or process solutions at 45°

C. with continuous agitation in the presence of approximately 1% by wt yeast extract, approximately 1% by weight mixed glucose/sucrose, and approximately 1% by weight microcrystalline cellulose.

9. The method of claim 1, wherein said slurry of bio-degradable components comprises dissolved solids and wherein at least 40% by weight of the dissolved volatile solids comprises lactate and/or wherein at least 40% by weight of the non-water content of the slurry of bio-degradable components comprises dissolved volatile solids.

10. The method of claim 1, wherein the microbial fermentation is conducted within the temperature range 45-50 degrees C.

11. The method of claim 1, wherein the unsorted MSW was heated to a temperature not higher than 75 degrees C.

12. The method of claim 1, wherein the removed non-biodegradable solids comprise at least 20% of the dry weight of the MSW, and/or wherein the removed non-biodegradable solids comprise at least 20% by dry weight of recyclable materials.

13. The method of claim 1, wherein removal of the non-biodegradable solids is performed within 36 hours from the start of enzymatic hydrolysis.

14. The method of claim 1, wherein ethanol or lactate are first removed from the slurry of bio-degradable components prior to anaerobic digestion to produce biomethane.