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Recombinant polymerases with increased phototolerance

  • US 10,358,634 B2
  • Filed: 08/04/2017
  • Issued: 07/23/2019
  • Est. Priority Date: 02/01/2012
  • Status: Active Grant
First Claim
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1. A composition comprising a ϕ

  • 29-type (phi29-type) recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 85% identical to SEQ ID NO;

    2, and which recombinant polymerase comprises one or more mutation selected from the group consisting of an amino acid substitution at position D17, an amino acid substitution at position P305, a Q96W substitution, a K128S substitution, an R258Q substitution, an K303Q substitution, a C452A substitution, an E463K substitution, an E463R substitution, and an E505Q substitution, wherein identification of positions is relative to SEQ ID NO;

    2, and wherein said polymerase exhibits polymerase activity.

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