Treatment of erythropoietin (EPO) related diseases by inhibition of natural antisense transcript to EPO
First Claim
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1. A method of identifying at least one modified oligonucleotide of 10 to 30 nucleotides in length having upregulatory activity for a target EPO polynucleotide having SEQ ID NO:
- 1;
determining oligonucleotides 10 to 30 consecutive nucleotides in length which are at least 95 percent complementary to an antisense polynucleotide of the EPO polynucleotide;
measuring the thermal melting point of a hybrid of said modified antisense oligonucleotide and the antisense polynucleotide of said EPO polynucleotide under stringent hybridization conditions; and
, measuring the upregulatory activity of said modified oligonucleotides by measuring EPO mRNA expression relative to a mock-transfected control.
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Abstract
Oligonucleotide compounds modulate expression and/or function of Erythropoietin (EPO) polynucleotides and encoded products thereof. Methods for treating diseases associated with Erythropoietin (EPO) comprise administering one or more oligonucleotide compounds designed to inhibit the EPO natural antisense transcript to patients.
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10 Claims
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1. A method of identifying at least one modified oligonucleotide of 10 to 30 nucleotides in length having upregulatory activity for a target EPO polynucleotide having SEQ ID NO:
- 1;
determining oligonucleotides 10 to 30 consecutive nucleotides in length which are at least 95 percent complementary to an antisense polynucleotide of the EPO polynucleotide;
measuring the thermal melting point of a hybrid of said modified antisense oligonucleotide and the antisense polynucleotide of said EPO polynucleotide under stringent hybridization conditions; and
, measuring the upregulatory activity of said modified oligonucleotides by measuring EPO mRNA expression relative to a mock-transfected control. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10)
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2. A method of identifying at least one active modified antisense oligonucleotide of 10 to 30 nucleotides in length which specifically hybridizes to a natural antisense polynucleotide of a EPO polynucleotide having SEQ ID NO:
- 1 and upregulates expression of said EPO polynucleotide comprising;
sequencing at least one antisense polynucleotide of the target EPO gene as a putative target; identifying at least one modified antisense oligonucleotide which is 100 percent complementary to the antisense polynucleotide target;
measuring the thermal melting point of a hybrid of said at least one modified antisense oligonucleotide and the target antisense polynucleotide under stringent hybridization conditions; and
measuring the upregulatory activity of said at least one modified oligonucleotide.
- 1 and upregulates expression of said EPO polynucleotide comprising;
Specification