Targeted modification of malate dehydrogenase
First Claim
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1. A tomato plant cell comprising a homozygously modified endogenous mitochondrial malate dehydrogenase (mMDH) gene such that activity of the expressed mMDH protein is reduced in the plant cell, wherein(i) the endogenous mMDH comprises sequences as shown in SEQ ID NO:
- 3-10;
(ii) the modification is made following cleavage by a nuclease comprising a cleavage domain and a DNA-binding domain that binds to a target site within any one of SEQ ID NO;
3-10; and
(iii) the modification is within or between SEQ ID NO;
3 and SEQ ID NO;
4;
within or between SEQ ID NO;
5 and SEQ ID NO;
6;
within or between SEQ ID NO;
7 and SEQ ID NO;
8;
or within or between SEQ ID NO;
9 and SEQ ID NO;
10.
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Abstract
Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.
50 Citations
16 Claims
-
1. A tomato plant cell comprising a homozygously modified endogenous mitochondrial malate dehydrogenase (mMDH) gene such that activity of the expressed mMDH protein is reduced in the plant cell, wherein
(i) the endogenous mMDH comprises sequences as shown in SEQ ID NO: - 3-10;
(ii) the modification is made following cleavage by a nuclease comprising a cleavage domain and a DNA-binding domain that binds to a target site within any one of SEQ ID NO;
3-10; and(iii) the modification is within or between SEQ ID NO;
3 and SEQ ID NO;
4;
within or between SEQ ID NO;
5 and SEQ ID NO;
6;
within or between SEQ ID NO;
7 and SEQ ID NO;
8;
or within or between SEQ ID NO;
9 and SEQ ID NO;
10. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- 3-10;
Specification