Arrangement and method for the electrochemical analysis of liquid samples by means of lateral flow assays

• US 10,359,388 B2
• Filed: 01/21/2013
• Issued: 07/23/2019
• Est. Priority Date: 02/08/2012
• Status: Active Grant

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Gumbrecht, Walter, Paulicka, Peter
• Primary Examiner(s): Leong, Susan D
• Application Number:
  US14/377,765
• Publication Number:
  US 20150008144A1
• Time in Patent Office: 2,374 Days
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/40 Semi-permeable membranes or partitions
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence

Abstract

An arrangement and to a method are provided for the electrical detection of liquid samples by lateral flow assays. The lateral flow assay includes a membrane arranged on a front side of a first carrier. The first carrier is electrically insulating. On the front side of the first carrier between the carrier and the membrane, electrically conductive electrodes are arranged in direct contact with the membrane.

Claims

1. An arrangement for electrical detection of liquid samples by lateral flow assays, the arrangement comprising:

a lateral flow assay comprising;

a first carrier configured in an electrically insulating fashion;

a membrane;

electrically conductive electrodes arranged on a front side of the first carrier, between the first carrier and the membrane, and in direct contact with the membrane; and

an electrode arranged on a rear side of a second carrier,

wherein the electrically conductive electrodes are arranged in a series in tandem or in array form on the front side of the first carrier,

wherein the membrane is arranged in a sandwich-like fashion between the front side of the first carrier in direct contact with the electrically conductive electrodes of the first carrier and the rear side of the second carrier in direct contact with the electrode on the rear side of the second carrier, and

wherein each electrode of the electrically conductive electrodes on the front side of the first carrier is electrically connected to the same electrode on the rear side of the second carrier.

2. The arrangement of claim 1, wherein the membrane is a closed layer via which the electrically conductive electrodes arranged on the front side of the first carrier are connected to one another.

3. The arrangement of claim 1, wherein the membrane comprises a lateral flow paper.

4. The arrangement of claim 1, wherein the electrically conductive electrodes on the front side of the first carrier and the electrode on the rear side of the second carrier are metal electrodes.

5. The arrangement of claim 1, wherein the electrically conductive electrodes on the front side of the first carrier are electrically contact-connected on the front side of the first carrier.

6. The arrangement of claim 1, wherein the first carrier has in each case in a region of a respective electrode of the electrically conductive electrodes on the front side of the first carrier, an opening passing through a thickness of the first carrier, from the front side to a rear side, the electrode being electrically contact-connected conductively from the front side to the rear side of the first carrier through the opening.

7. The arrangement of claim 1, wherein the first carrier comprises a plurality of insulating layers.

8. The arrangement of claim 3, wherein the membrane comprises a lateral flow paper composed of nitrocellulose.

9. The arrangement of claim 4, wherein the electrically conductive electrodes on the front side of the first carrier and the electrode on the rear side of the second carrier are metal electrodes composed of gold.

10. The arrangement of claim 5, wherein the electrically conductive electrodes on the front side of the first carrier are electrically contact-connected on the front side of the first carrier in an edge region in which the membrane is not arranged.

11. The arrangement of claim 7, wherein the insulating layers are composed of polymers, are connected to one another by lamination, or are composed of polymers and are connected to one another by lamination.

12. The arrangement of claim 1, wherein each electrode of the electrically conductive electrodes on the front side of the first carrier is electrically connected to the electrode on the rear side of the second carrier via an electrical measuring device for measuring current, voltage, capacitance, or any combination thereof.

13. The arrangement of claim 1, wherein capture molecules are immobilized on the electrically conductive electrodes on the front side of the first carrier.

14. The arrangement of claim 1, wherein the electrically conductive electrodes on the front side of the first carrier are configured to produce a spatially resolved electrochemical measurement of the liquid samples.