Portable sampling device and method for detection of biomarkers in exhaled breath
First Claim
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1. A method of detecting biomarkers from exhaled breath of a subject, comprising:
- collecting a sample of aerosols from a deep breath reaching exhaled breath from central or peripheral lung regions in a sampling membrane arranged in a handheld and portable collection device, the collection device includes a chamber made from two parts jointed together for the exhaled breath to be exhaled through, wherein the sampling membrane comprises at least two layers, and wherein at least one layer is gas permeable and another layer is an electrostatic non-woven filter, the sampling membrane is arranged transversal to a flow of exhaled breath in the chamber to collect said aerosols from the exhaled breath, and wherein said sampling membrane is configured for a low pressure drop through said handheld and portable collection device for said subject to exhale through said handheld and portable device un-aided, so that said exhaled breath flows unaided from an inlet to an outlet of said device;
the device further comprises a detachable tubular element having a lumen in flow connection with the inlet of the collection device;
wherein the tubular element is formed by two opposite parts, each part having at least one baffle such that when connected the tubular element comprises at least two baffles arranged on opposite sides of said lumen to provide a non-straight path through the tubular element, said baffles protrude into said lumen at a distance larger than a radius of said lumen;
the device further comprises a volume measure unit for determining that a pre-defined volume of said exhaled breath has passed through said sampling membrane, wherein the volume measure unit is connected through a port in the tubular element, and wherein said volume measuring unit is a non-elastic bag with a predetermined volume;
sealing said inlet and said outlet by mounting plugs in the inlet and the outlet for transportation;
extracting a content from said sampling membrane, wherein said content is extracted from said sampling membrane using a solvent; and
employing a sensor unit for detecting traces of biomarkers in said content, wherein said sensor unit performs an analyzing method based on liquid chromatography mass-spectroscopy (LC/MS).
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Abstract
A portable method and sampling device for collecting aerosols comprising biomarkers from exhaled breath of a subject for further sensor based analysis. The sampling device (41) comprising a housing (406) comprising at least one inlet (407) and at least one outlet (408) for the exhaled breath to exit through, and a sampling membrane arranged in the housing. The sampling membrane is arranged to collect the aerosols from said exhaled breath.
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Citations
23 Claims
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1. A method of detecting biomarkers from exhaled breath of a subject, comprising:
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collecting a sample of aerosols from a deep breath reaching exhaled breath from central or peripheral lung regions in a sampling membrane arranged in a handheld and portable collection device, the collection device includes a chamber made from two parts jointed together for the exhaled breath to be exhaled through, wherein the sampling membrane comprises at least two layers, and wherein at least one layer is gas permeable and another layer is an electrostatic non-woven filter, the sampling membrane is arranged transversal to a flow of exhaled breath in the chamber to collect said aerosols from the exhaled breath, and wherein said sampling membrane is configured for a low pressure drop through said handheld and portable collection device for said subject to exhale through said handheld and portable device un-aided, so that said exhaled breath flows unaided from an inlet to an outlet of said device;
the device further comprises a detachable tubular element having a lumen in flow connection with the inlet of the collection device;
wherein the tubular element is formed by two opposite parts, each part having at least one baffle such that when connected the tubular element comprises at least two baffles arranged on opposite sides of said lumen to provide a non-straight path through the tubular element, said baffles protrude into said lumen at a distance larger than a radius of said lumen;
the device further comprises a volume measure unit for determining that a pre-defined volume of said exhaled breath has passed through said sampling membrane, wherein the volume measure unit is connected through a port in the tubular element, and wherein said volume measuring unit is a non-elastic bag with a predetermined volume;sealing said inlet and said outlet by mounting plugs in the inlet and the outlet for transportation; extracting a content from said sampling membrane, wherein said content is extracted from said sampling membrane using a solvent; and employing a sensor unit for detecting traces of biomarkers in said content, wherein said sensor unit performs an analyzing method based on liquid chromatography mass-spectroscopy (LC/MS). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A portable sampling device for collecting aerosols comprising biomarkers from exhaled breath of a subject for further sensor based analysis, comprising:
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a housing configured to be handheld comprising at least one inlet and at least one outlet for said exhaled breath to exit through, the housing includes a chamber made from two parts jointed together for the exhaled breath to be exhaled through and a sampling membrane comprising at least two layers, wherein at least one layer is gas permeable and another layer is an electrostatic non-woven filter, the sampling membrane being arranged transversal to a flow of exhaled breath in the chamber to collect said aerosols from the exhaled breath;
plugs to be mounted in the inlet and the outlet for transportation of the device; andwherein said sampling membrane is a fiber based filter membrane, wherein said filter membrane comprises at least one layer of non-woven filtration media arranged transversal to the flow of exhaled breath in said chamber to collect said aerosols from said exhaled breath and wherein said sampling membrane is configured for a low pressure drop through said portable sampling device for allowing said subject to exhale through said handheld and portable device un-aided from the inlet to the outlet of said device; and
wherein a content in said sampling membrane is extractable from said sampling membrane using a solvent for analysis with liquid chromatography-mass-spectroscopy;wherein the device further comprises a detachable tubular element having a lumen in flow connection with the inlet to the chamber;
wherein the tubular element is formed by two opposite parts, each part having at least one baffle, which when connected comprises at least two baffles arranged on opposite sides of said lumen to provide a non-straight path through the tubular element, said baffles protrude into said lumen at a distance larger than a radius of said lumen;
the device further comprises a volume measure unit for determining that a pre-defined volume of said exhaled breath has passed through said sampling membrane, wherein the volume measure unit is connected through a port in the tubular element; and
wherein said volume measure unit is a non-elastic bag with a predetermined volume. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification