Histological specimen treatment

• US 10,365,189 B2
• Filed: 05/07/2015
• Issued: 07/30/2019
• Est. Priority Date: 05/07/2015
• Status: Active Grant

• Current Assignee: Steven Wheeler
• Original Assignee: Steven Wheeler
• Inventors: Wheeler, Steven
• Primary Examiner(s): Kim, Taeyoon
• Assistant Examiner(s): Gough, Tiffany M
• Application Number:
  US14/706,389
• Publication Number:
  US 20160327457A1
• Time in Patent Office: 1,545 Days
• CPC Class Codes: C08F 220/20 of polyhydric alcohols or phenols , e.g. 2-hydroxyethyl (meth)acrylate or glycerol mono-(meth)acrylate
• CPC Class Codes: C08F 220/281 and containing only one oxygen, e.g. furfuryl (meth)acrylate or 2-methoxyethyl (meth)acrylate
• CPC Class Codes: C08F 222/102 of dialcohols, e.g. ethylene glycol di(meth)acrylate or 1,4-butanediol dimethacrylate
• CPC Class Codes: C08F 2800/20 as weight or mass percentages
• CPC Class Codes: C08K 5/0016 Plasticisers
• CPC Class Codes: C08K 5/12 of cyclic polycarboxylic acids
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 2001/307 non-toxic, no Hg, no formaldehyde

Abstract

A single dissolving compound forms plural azeotropes, which can be azeotropically vaporized off at various stages of the treatment process, thus maintaining predictable concentrations of the chemicals present. The treatment process can be performed in the absence of formalin or related compounds which can interfere with the preservation of genetic material. A process for preserving a specimen includes using a dissolving compound that can form a plural number of azeotropes, at least one azeotrope being formed between one or more components of the dissolving compound and specimen-supplied water, and at least one azeotrope being formed between different components of the dissolving compound; successively and azeotropically vaporizing off formed azeotropes; and impregnating the specimen with a support medium.

Claims

1. A process for preserving a specimen comprising:

using a dissolving compound that can form a plural number of azeotropes;

successively and azeotropically vaporizing off the formed azeotropes, the first formed azeotrope being vaporized off by reducing pressure, followed by raising temperature to form the second azeotrope, the second azeotrope being vaporized off by reducing pressure, followed by raising temperature to form the third azeotrope, and the third azeotrope being vaporized off by reducing pressure;

wherein a first azeotrope is formed between one or more components of the dissolving compound and specimen-supplied water, a second azeotrope is formed between different components of the dissolving compound, and a third azeotrope is formed between different components of the dissolving compound, the third azeotrope being different from the second azeotrope; and

impregnating the specimen with a support medium.

2. The process of claim 1, wherein the successively and azeotropically vaporizing off the azeotropes occurs at successively increasing boiling point temperatures.

3. The process of claim 1, wherein the azeotropes form at successively higher temperatures.

4. The process of claim 1, wherein the dissolving compound comprises methanol, tetrahydrofuran, toluene, and zinc acetate.

5. The process of claim 1, wherein the azeotropes formed comprise tetrahydrofuran-methanol, toluene-methanol, tetrahydrofuran-water, and toluene-water.

11. The process of claim 1, wherein the dissolving compounds performs fixation, dehydration and clearing.

12. The process of claim 11, wherein the dissolving compounds facilitate infiltration of the support medium.

6. A process for preserving a specimen comprises:

dissolving and removing cellular solutes in the specimen using a dissolving compound;

partially removing the dissolving compound by forming two or more different azeotropes, at least two of the different azeotropes being formed with at least two component agents of the dissolving compound, and azeotropically vaporizing off the two or more different azeotropes, a first of the two or more different azeotropes being vaporized off by reducing pressure, followed by raising temperature to form a second of the two or more different azeotropes, the second of the two or more different azeotropes being vaporized off by reducing pressure; and

replacing the solutes with an impregnated support medium.

7. The process of claim 6, performed in a continuous sequence of steps within a single vessel.

8. The process of claim 6, performed in the absence of formalin.

9. The process of claim 6, wherein the impregnated support medium comprises paraffin.

10. The process of claim 6, wherein the impregnated support medium is selected from the group consisting of:

paraffin, plastic polymers, polyvinyl alcohol, polyethylene glycols, waxes, cellulose derivatives, agars, gels, and sugars.

13. The process of claim 6, wherein the dissolving compounds performs fixation, dehydration and clearing.

14. The process of claim 13, wherein the dissolving compounds facilitate infiltration of the support medium.