Digital microfluidic dilution apparatus, systems, and related methods

• US 10,369,565 B2
• Filed: 12/21/2015
• Issued: 08/06/2019
• Est. Priority Date: 12/31/2014
• Status: Active Grant

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Fischer, Andrew
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US14/976,684
• Publication Number:
  US 20160184823A1
• Time in Patent Office: 1,324 Days
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension

Abstract

Example methods, apparatus, systems for diluting samples are disclosed. An example method includes depositing a first fluid droplet on a first electrode of a plurality of electrodes. The first electrode has a first area. The first fluid droplet has a first volume associated with the first area. The example method includes depositing a second fluid droplet on a second electrode of the plurality of electrodes. The second electrode has a second area. The second fluid droplet has a second volume associated with the second area. The second volume is different than the first volume. The example method includes forming a combined droplet by selectively activating at least one of the first electrode or the second electrode to cause one of the first fluid droplet or the second fluid droplet to merge with the other of the first fluid droplet or the second fluid droplet.

Claims

1. A method comprising:

patterning a first electrode on a first substrate, the first electrode having a first area;

patterning a second electrode on the first substrate, the second electrode having a second area, wherein the second area is a first fraction of the first area;

patterning a third electrode on the first substrate, the third electrode having a third area, wherein the third area is a second fraction of the first area;

associating the first electrode with a first volume based on the first area and a height of a gap between the first substrate and a second substrate; and

associating the second electrode with a second volume based on the second area and the height of the gap;

associating the third electrode with a third volume based on the third area and the height of the gap, wherein the first electrode, the second electrode, and the third electrode are represented in a binary sequence based on the first area and the first volume, the second area and the second volume, and the third area and the third volume, respectively, and wherein the first electrode, the second electrode, and the third electrode are disposed non-linearly on the first substrate.

2. The method of claim 1, further including coating the first electrode, the second electrode, or the third electrode with at least one of hydrophobic or dielectric material.

3. The method of claim 1, further including patterning the first electrode the second electrode, or the third electrode on the first substrate using one or more of a laser or a photolithographic printer.

4. The method of claim 1, further including calculating the binary sequence for a plurality of electrodes with respect to first area of the first electrode.

5. The method of claim 1, wherein patterning the second electrode further includes printing the second electrode to have an asymmetric shape.

6. The method of claim 1, further including patterning a fourth electrode on the first substrate to interlock with the first electrode.

7. The method of claim 1, further including arranging the first electrode, the second electrode, and the third electrode on the first substrate based on an area of the first substrate.

8. An apparatus comprising:

a first substrate;

a second substrate, the second substrate spaced apart from the first substrate;

an electrode pattern disposed on the first substrate, the electrode pattern including a plurality of electrodes including a first electrode having a first area, a second electrode having a first fractional area relative to the first area, and a third electrode having a second fractional area relative to the first area, each of the first area, the first fractional area, and the second fractional area being different, wherein the first electrode, the second electrode, and the third electrode are disposed non-linearly on the first substrate.

9. The apparatus of claim 8, wherein the first fractional area is one-half of the first area.

10. The apparatus of claim 8, wherein the second fractional area is one-fourth of the first area.

11. The apparatus of claim 8, wherein the electrode pattern further comprises a fourth electrode having a third fractional area relative to the first area.

12. The apparatus of claim 11, wherein the electrode pattern further comprises a fifth electrode having a fourth fractional area relative to the first area.

13. The apparatus of claim 11, wherein the third fractional area is substantially equal to one of the first fractional area or the second fractional area.

14. The apparatus of claim 11, wherein the third fractional area is different than the first fractional area and the second fractional area.

15. The apparatus of claim 8, wherein the first area is associated with a first volume of a first droplet to be disposed on the first electrode, the first fractional area is associated with a second volume of a second droplet to be disposed on the second electrode, and the second fractional area is associated with a third volume of a third droplet to be disposed on the third electrode, the second and third volumes being fractional volumes relative to the first volume based on the electrode pattern.

16. The apparatus of claim 15, wherein the second volume is substantially equal to one-half of the first volume.