Devices and methods for overlaying blood or cellular suspensions
First Claim
1. A trap insert configured for insertion into a conical container, the trap insert comprising:
- a conical opening;
a lip;
a reservoir defined by a partial wall;
an exit chamber;
a cap, the cap comprising;
a flat circular member; and
hooks extending upwards from edges of the circular member.
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Accused Products
Abstract
A device is described that overlays a first fluid, such as blood or a cellular suspension onto a base material, such as a density gradient. In some embodiments, the fluid layering device includes a cylindrical reservoir, a fluid barrier, a coupling extension, a plunger, and an exhaust vent. The fluid layering device can be coupled through its coupling extension to an open end of a container, such as a conical centrifuge tube, including the density gradient. Once attached, the plunger may be lowered to a position above the surface of the density gradient. A first fluid may flow from the reservoir into the conical tube across the plunger, so that a suitable overlay is formed without substantially disturbing a surface of the density gradient. The plunger may be buoyed upward by the first fluid during operation, providing a constant regulation of flow throughout overlaying, regardless of the care and skill of the user.
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Citations
9 Claims
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1. A trap insert configured for insertion into a conical container, the trap insert comprising:
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a conical opening; a lip; a reservoir defined by a partial wall; an exit chamber; a cap, the cap comprising; a flat circular member; and hooks extending upwards from edges of the circular member. - View Dependent Claims (2, 3, 4, 5)
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6. A system comprising:
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an open-ended container; and a fluid layering device configured control a flow of fluid into the open-ended container, the fluid layering device comprising a trap member inserted into the open-ended container, the trap member comprising; a conical opening; a lip; a reservoir defined by a partial wall; an exit chamber; a cap, the cap comprising; a flat circular member; and hooks extending upwards from edges of the circular member. - View Dependent Claims (7, 8, 9)
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Specification