Pluripotent stem cell that induces repair and regeneration after myocardial infarction
First Claim
1. A method of treating myocardial infarction in a subject in need thereof, the method comprising:
- administering to said subject a cell preparation comprising isolated pluripotent stem cells positive for SSEA-3, wherein the pluripotent stem cells are isolated from a mesenchymal cell population obtained from mesenchymal tissue or cultured mesenchymal cells, wherein said pluripotent stem cells are isolated by subjecting said mesenchymal cell population to external stress stimulation or cell sorting, to thereby treat the myocardial infarction, and wherein the pluripotent stem cells have a plurality of properties comprising;
(i) CD105-positivity;
(ii) low or absent telomerase activity;
(iii) ability to differentiated into embryonic endoderm, ectoderm, and mesoderm germ layers;
(iv) absence of neoplastic proliferation; and
(v) ability to self-renew.
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Accused Products
Abstract
An object of the present invention is to provide a novel medical application for use in regenerative medicine that uses pluripotent stem cells (Muse cells). The present invention provides a cell preparation for treating myocardial infarction, and particularly serious massive myocardial infarction and heart failure associated therewith, that contains pluripotent stem cells positive for SSEA-3 isolated from biological mesenchymal tissue or cultured mesenchymal cells. The cell preparation of the present invention is based on a cardiac tissue regeneration mechanism by which Muse cells are made to selectively accumulate in damaged myocardial tissue and differentiate into cardiac muscle in that tissue as a result of intravenous administration of Muse cells to a subject presenting with the aforementioned disorders.
5 Citations
14 Claims
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1. A method of treating myocardial infarction in a subject in need thereof, the method comprising:
- administering to said subject a cell preparation comprising isolated pluripotent stem cells positive for SSEA-3, wherein the pluripotent stem cells are isolated from a mesenchymal cell population obtained from mesenchymal tissue or cultured mesenchymal cells, wherein said pluripotent stem cells are isolated by subjecting said mesenchymal cell population to external stress stimulation or cell sorting, to thereby treat the myocardial infarction, and wherein the pluripotent stem cells have a plurality of properties comprising;
(i) CD105-positivity;
(ii) low or absent telomerase activity;
(iii) ability to differentiated into embryonic endoderm, ectoderm, and mesoderm germ layers;
(iv) absence of neoplastic proliferation; and
(v) ability to self-renew. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- administering to said subject a cell preparation comprising isolated pluripotent stem cells positive for SSEA-3, wherein the pluripotent stem cells are isolated from a mesenchymal cell population obtained from mesenchymal tissue or cultured mesenchymal cells, wherein said pluripotent stem cells are isolated by subjecting said mesenchymal cell population to external stress stimulation or cell sorting, to thereby treat the myocardial infarction, and wherein the pluripotent stem cells have a plurality of properties comprising;
Specification